Platelet-neutrophil interaction and superoxide anion generation

Involvement of purine nucleotides

Susanna Colli, Sonia Eligini, Mariagrazia Lalli, Elena Tremoli

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Stimulated platelets secrete a variety of physiologically active substances that affect many neutrophil functions. We have examined the capacity of platelets to modulate superoxide anion generation by neutrophils. The amounts of superoxide anion produced by neutrophils in the presence of platelets were markedly enhanced when platelet-neutrophil coincubations were stimulated with agents that simultaneously activate both cell types, as the calcium ionophore A23187 and sodium arachidonate. This effect was dependent upon the number of platelets added to the incubation media and was not affected by inhibitors of arachidonic acid pathway or by preincubation of platelets with an antibody anti-P-selectin. The hypothesis of an involvement of purine nucleotides released by platelets during aggregation on the observed effect of enhancement of superoxide anion generation by neutrophils was then tested. Experimental evidence indicates that platelets release, during A23187-induced aggregation, amounts of ATP that are of the same order (5-10 μM) of those demonstrated to enhance superoxide anion generation by neutrophils. In addition, platelet lysates mimicked the effect of intact platelets in enhancing superoxide anion generation by A23187 stimulated neutrophils. Interestingly, at variance with the results obtained with intact platelets and platelet lysates, supernatants of thrombin-stimulated platelets did not increase O2·- by neutrophils. The enhancing effect of these supernatants was, however, restored when platelets were preincubated with an antibody anti P-selectin. These data indicate that platelets, through the release of purine nucleotides, enhance superoxide generation by neutrophils, thus increasing the cytotoxic potential of these cells.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalFree Radical Biology and Medicine
Volume20
Issue number3
DOIs
Publication statusPublished - 1996

Fingerprint

Purine Nucleotides
Platelets
Superoxides
Neutrophils
Blood Platelets
Calcimycin
P-Selectin
Arachidonic Acid
Anti-Idiotypic Antibodies
Agglomeration
Calcium Ionophores
Platelet Count
Platelet Aggregation
Thrombin

Keywords

  • Cell-cell interaction
  • Free radicals
  • Neutrophils
  • P-Selectin
  • Platelets
  • Purine nucleotides
  • Superoxide anion

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Clinical Biochemistry

Cite this

Platelet-neutrophil interaction and superoxide anion generation : Involvement of purine nucleotides. / Colli, Susanna; Eligini, Sonia; Lalli, Mariagrazia; Tremoli, Elena.

In: Free Radical Biology and Medicine, Vol. 20, No. 3, 1996, p. 271-278.

Research output: Contribution to journalArticle

@article{9a24aa1d886640e7b1367c196a1e5bb4,
title = "Platelet-neutrophil interaction and superoxide anion generation: Involvement of purine nucleotides",
abstract = "Stimulated platelets secrete a variety of physiologically active substances that affect many neutrophil functions. We have examined the capacity of platelets to modulate superoxide anion generation by neutrophils. The amounts of superoxide anion produced by neutrophils in the presence of platelets were markedly enhanced when platelet-neutrophil coincubations were stimulated with agents that simultaneously activate both cell types, as the calcium ionophore A23187 and sodium arachidonate. This effect was dependent upon the number of platelets added to the incubation media and was not affected by inhibitors of arachidonic acid pathway or by preincubation of platelets with an antibody anti-P-selectin. The hypothesis of an involvement of purine nucleotides released by platelets during aggregation on the observed effect of enhancement of superoxide anion generation by neutrophils was then tested. Experimental evidence indicates that platelets release, during A23187-induced aggregation, amounts of ATP that are of the same order (5-10 μM) of those demonstrated to enhance superoxide anion generation by neutrophils. In addition, platelet lysates mimicked the effect of intact platelets in enhancing superoxide anion generation by A23187 stimulated neutrophils. Interestingly, at variance with the results obtained with intact platelets and platelet lysates, supernatants of thrombin-stimulated platelets did not increase O2·- by neutrophils. The enhancing effect of these supernatants was, however, restored when platelets were preincubated with an antibody anti P-selectin. These data indicate that platelets, through the release of purine nucleotides, enhance superoxide generation by neutrophils, thus increasing the cytotoxic potential of these cells.",
keywords = "Cell-cell interaction, Free radicals, Neutrophils, P-Selectin, Platelets, Purine nucleotides, Superoxide anion",
author = "Susanna Colli and Sonia Eligini and Mariagrazia Lalli and Elena Tremoli",
year = "1996",
doi = "10.1016/0891-5849(95)02032-2",
language = "English",
volume = "20",
pages = "271--278",
journal = "Free Radical Biology and Medicine",
issn = "0891-5849",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Platelet-neutrophil interaction and superoxide anion generation

T2 - Involvement of purine nucleotides

AU - Colli, Susanna

AU - Eligini, Sonia

AU - Lalli, Mariagrazia

AU - Tremoli, Elena

PY - 1996

Y1 - 1996

N2 - Stimulated platelets secrete a variety of physiologically active substances that affect many neutrophil functions. We have examined the capacity of platelets to modulate superoxide anion generation by neutrophils. The amounts of superoxide anion produced by neutrophils in the presence of platelets were markedly enhanced when platelet-neutrophil coincubations were stimulated with agents that simultaneously activate both cell types, as the calcium ionophore A23187 and sodium arachidonate. This effect was dependent upon the number of platelets added to the incubation media and was not affected by inhibitors of arachidonic acid pathway or by preincubation of platelets with an antibody anti-P-selectin. The hypothesis of an involvement of purine nucleotides released by platelets during aggregation on the observed effect of enhancement of superoxide anion generation by neutrophils was then tested. Experimental evidence indicates that platelets release, during A23187-induced aggregation, amounts of ATP that are of the same order (5-10 μM) of those demonstrated to enhance superoxide anion generation by neutrophils. In addition, platelet lysates mimicked the effect of intact platelets in enhancing superoxide anion generation by A23187 stimulated neutrophils. Interestingly, at variance with the results obtained with intact platelets and platelet lysates, supernatants of thrombin-stimulated platelets did not increase O2·- by neutrophils. The enhancing effect of these supernatants was, however, restored when platelets were preincubated with an antibody anti P-selectin. These data indicate that platelets, through the release of purine nucleotides, enhance superoxide generation by neutrophils, thus increasing the cytotoxic potential of these cells.

AB - Stimulated platelets secrete a variety of physiologically active substances that affect many neutrophil functions. We have examined the capacity of platelets to modulate superoxide anion generation by neutrophils. The amounts of superoxide anion produced by neutrophils in the presence of platelets were markedly enhanced when platelet-neutrophil coincubations were stimulated with agents that simultaneously activate both cell types, as the calcium ionophore A23187 and sodium arachidonate. This effect was dependent upon the number of platelets added to the incubation media and was not affected by inhibitors of arachidonic acid pathway or by preincubation of platelets with an antibody anti-P-selectin. The hypothesis of an involvement of purine nucleotides released by platelets during aggregation on the observed effect of enhancement of superoxide anion generation by neutrophils was then tested. Experimental evidence indicates that platelets release, during A23187-induced aggregation, amounts of ATP that are of the same order (5-10 μM) of those demonstrated to enhance superoxide anion generation by neutrophils. In addition, platelet lysates mimicked the effect of intact platelets in enhancing superoxide anion generation by A23187 stimulated neutrophils. Interestingly, at variance with the results obtained with intact platelets and platelet lysates, supernatants of thrombin-stimulated platelets did not increase O2·- by neutrophils. The enhancing effect of these supernatants was, however, restored when platelets were preincubated with an antibody anti P-selectin. These data indicate that platelets, through the release of purine nucleotides, enhance superoxide generation by neutrophils, thus increasing the cytotoxic potential of these cells.

KW - Cell-cell interaction

KW - Free radicals

KW - Neutrophils

KW - P-Selectin

KW - Platelets

KW - Purine nucleotides

KW - Superoxide anion

UR - http://www.scopus.com/inward/record.url?scp=0029980677&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029980677&partnerID=8YFLogxK

U2 - 10.1016/0891-5849(95)02032-2

DO - 10.1016/0891-5849(95)02032-2

M3 - Article

VL - 20

SP - 271

EP - 278

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

SN - 0891-5849

IS - 3

ER -