Platelet proteome in healthy volunteers who smoke

Anna Della Corte, Chiara Tamburrelli, Marilena Crescente, Lucia Giordano, Marco D'Imperio, Michela Di Michele, Maria Benedetta Donati, Giovanni De Gaetano, Domenico Rotilio, Chiara Cerletti

Research output: Contribution to journalArticle

Abstract

Smoking accelerates atherosclerosis and is a well-known risk factor for acute cardiovascular complications; however, the mechanisms of these effects have not been completely clarified. Recently developed proteomic approaches may offer new clues when combined with well-established functional tests. Platelet proteome of healthy smokers and non-smokers was resolved by two-dimensional difference gel electrophoresis, compared by Decyder software and identified by mass spectrometry analysis (nano-LC-MS/MS). In smokers, three proteins (Factor XIII-A subunit, platelet glycoprotein IIb and beta-actin) were significantly up-regulated, whereas WDR1 protein and chaperonine HSP60 were down-regulated. Furthermore, the highest scored network derived by Ingenuity Pathway Analysis using the modulated proteins as input showed the involvement of several proteins to be related to inflammation and apoptosis. Platelet function tests and the levels of markers of platelet and leukocyte activation were not different in smokers vs. non-smoker subjects. The platelet proteomic approach confirms that cigarette smoking triggers several inflammatory reactions and may help clarify some of the molecular mechanisms of smoke effect on cellular systems relevant for vascular integrity and human health.

Original languageEnglish
Pages (from-to)91-105
Number of pages15
JournalPlatelets
Volume23
Issue number2
DOIs
Publication statusPublished - Mar 2012

Fingerprint

Proteome
Smoke
Healthy Volunteers
Blood Platelets
Proteomics
Proteins
Platelet Function Tests
Smoking
Two-Dimensional Difference Gel Electrophoresis
Platelet Membrane Glycoproteins
Factor XIII
Platelet Activation
Blood Vessels
Actins
Mass Spectrometry
Atherosclerosis
Leukocytes
Software
Apoptosis
Inflammation

Keywords

  • DIGE
  • factor XIII
  • mass spectrometry
  • pathway analysis
  • platelet proteome
  • Smoke

ASJC Scopus subject areas

  • Hematology

Cite this

Della Corte, A., Tamburrelli, C., Crescente, M., Giordano, L., D'Imperio, M., Di Michele, M., ... Cerletti, C. (2012). Platelet proteome in healthy volunteers who smoke. Platelets, 23(2), 91-105. https://doi.org/10.3109/09537104.2011.587916

Platelet proteome in healthy volunteers who smoke. / Della Corte, Anna; Tamburrelli, Chiara; Crescente, Marilena; Giordano, Lucia; D'Imperio, Marco; Di Michele, Michela; Donati, Maria Benedetta; De Gaetano, Giovanni; Rotilio, Domenico; Cerletti, Chiara.

In: Platelets, Vol. 23, No. 2, 03.2012, p. 91-105.

Research output: Contribution to journalArticle

Della Corte, A, Tamburrelli, C, Crescente, M, Giordano, L, D'Imperio, M, Di Michele, M, Donati, MB, De Gaetano, G, Rotilio, D & Cerletti, C 2012, 'Platelet proteome in healthy volunteers who smoke', Platelets, vol. 23, no. 2, pp. 91-105. https://doi.org/10.3109/09537104.2011.587916
Della Corte A, Tamburrelli C, Crescente M, Giordano L, D'Imperio M, Di Michele M et al. Platelet proteome in healthy volunteers who smoke. Platelets. 2012 Mar;23(2):91-105. https://doi.org/10.3109/09537104.2011.587916
Della Corte, Anna ; Tamburrelli, Chiara ; Crescente, Marilena ; Giordano, Lucia ; D'Imperio, Marco ; Di Michele, Michela ; Donati, Maria Benedetta ; De Gaetano, Giovanni ; Rotilio, Domenico ; Cerletti, Chiara. / Platelet proteome in healthy volunteers who smoke. In: Platelets. 2012 ; Vol. 23, No. 2. pp. 91-105.
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