Platelet reactivity and endothelial function in children of patients with early acute myocardial infarction

Gaetano A. Lanza, Giancarla Scalone, Lucy Barone, Fabio Infusino, Ilaria Coviello, Antonio Di Monaco, Angelica Delogu, Irma Battipaglia, Alessia De Nisco, Alfonso Sestito, Costantino Romagnoli, Filippo Crea

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

AimsTo assess whether platelet reactivity is increased in offspring of patients with early acute myocardial infarction (AMI) and its possible relation with endothelial dysfunction.Methods and resultsWe studied 23 healthy children (15 ± 3 years, 13 males) of patients with early AMI (≤50 years old; Group 1) and 21 healthy children of healthy subjects without any history of cardiovascular disease (14 ± 3 years, 10 males; Group 2). Platelet reactivity was assessed by flow cytometry as the increase in monocyte-platelet aggregates (MPA) and CD41 and PAC-1 platelet expression in response to exercise stress test (EST), adenosine diphosphate (ADP) stimulation (10 -7 M), or both. Endothelial function was assessed by measuring brachial artery dilation during post-ischaemic forearm hyperaemia [flow-mediated dilation (FMD)]. Both EST and ADP induced a higher percentage increase in platelet receptor expression in Group 1, compared with Group 2, with the most significant difference being shown for the response to the combined stimuli (e.g. MPA, 23.1 ± 12 vs. 5.63 ± 8, P <0.001; platelet PAC-1, 57.7 ± 47 vs. 13.2 ± 7, P <0.001). Compared with Group 2, Group 1 children showed lower FMD (10.7 ± 3.1 vs. 8.0 ± 2.9, respectively; P 0.007). However, no significant association was found between FMD and platelet reactivity. ConclusionOur results show increased platelet reactivity in children of patients with early AMI; the finding was not significantly correlated with endothelial dysfunction, suggesting that other mechanisms are mainly involved in the enhanced platelet response to agonistic stimuli.

Original languageEnglish
Pages (from-to)2042-2049
Number of pages8
JournalEuropean Heart Journal
Volume32
Issue number16
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Blood Platelets
Myocardial Infarction
Exercise Test
Dilatation
Adenosine Diphosphate
Monocytes
Brachial Artery
Hyperemia
Forearm
Healthy Volunteers
Flow Cytometry
Cardiovascular Diseases

Keywords

  • Endothelial dysfunction
  • Family history of acute myocardial infarction
  • Platelet aggregation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Platelet reactivity and endothelial function in children of patients with early acute myocardial infarction. / Lanza, Gaetano A.; Scalone, Giancarla; Barone, Lucy; Infusino, Fabio; Coviello, Ilaria; Di Monaco, Antonio; Delogu, Angelica; Battipaglia, Irma; De Nisco, Alessia; Sestito, Alfonso; Romagnoli, Costantino; Crea, Filippo.

In: European Heart Journal, Vol. 32, No. 16, 08.2011, p. 2042-2049.

Research output: Contribution to journalArticle

Lanza, GA, Scalone, G, Barone, L, Infusino, F, Coviello, I, Di Monaco, A, Delogu, A, Battipaglia, I, De Nisco, A, Sestito, A, Romagnoli, C & Crea, F 2011, 'Platelet reactivity and endothelial function in children of patients with early acute myocardial infarction', European Heart Journal, vol. 32, no. 16, pp. 2042-2049. https://doi.org/10.1093/eurheartj/ehr109
Lanza, Gaetano A. ; Scalone, Giancarla ; Barone, Lucy ; Infusino, Fabio ; Coviello, Ilaria ; Di Monaco, Antonio ; Delogu, Angelica ; Battipaglia, Irma ; De Nisco, Alessia ; Sestito, Alfonso ; Romagnoli, Costantino ; Crea, Filippo. / Platelet reactivity and endothelial function in children of patients with early acute myocardial infarction. In: European Heart Journal. 2011 ; Vol. 32, No. 16. pp. 2042-2049.
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abstract = "AimsTo assess whether platelet reactivity is increased in offspring of patients with early acute myocardial infarction (AMI) and its possible relation with endothelial dysfunction.Methods and resultsWe studied 23 healthy children (15 ± 3 years, 13 males) of patients with early AMI (≤50 years old; Group 1) and 21 healthy children of healthy subjects without any history of cardiovascular disease (14 ± 3 years, 10 males; Group 2). Platelet reactivity was assessed by flow cytometry as the increase in monocyte-platelet aggregates (MPA) and CD41 and PAC-1 platelet expression in response to exercise stress test (EST), adenosine diphosphate (ADP) stimulation (10 -7 M), or both. Endothelial function was assessed by measuring brachial artery dilation during post-ischaemic forearm hyperaemia [flow-mediated dilation (FMD)]. Both EST and ADP induced a higher percentage increase in platelet receptor expression in Group 1, compared with Group 2, with the most significant difference being shown for the response to the combined stimuli (e.g. MPA, 23.1 ± 12 vs. 5.63 ± 8, P <0.001; platelet PAC-1, 57.7 ± 47 vs. 13.2 ± 7, P <0.001). Compared with Group 2, Group 1 children showed lower FMD (10.7 ± 3.1 vs. 8.0 ± 2.9, respectively; P 0.007). However, no significant association was found between FMD and platelet reactivity. ConclusionOur results show increased platelet reactivity in children of patients with early AMI; the finding was not significantly correlated with endothelial dysfunction, suggesting that other mechanisms are mainly involved in the enhanced platelet response to agonistic stimuli.",
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AU - Lanza, Gaetano A.

AU - Scalone, Giancarla

AU - Barone, Lucy

AU - Infusino, Fabio

AU - Coviello, Ilaria

AU - Di Monaco, Antonio

AU - Delogu, Angelica

AU - Battipaglia, Irma

AU - De Nisco, Alessia

AU - Sestito, Alfonso

AU - Romagnoli, Costantino

AU - Crea, Filippo

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N2 - AimsTo assess whether platelet reactivity is increased in offspring of patients with early acute myocardial infarction (AMI) and its possible relation with endothelial dysfunction.Methods and resultsWe studied 23 healthy children (15 ± 3 years, 13 males) of patients with early AMI (≤50 years old; Group 1) and 21 healthy children of healthy subjects without any history of cardiovascular disease (14 ± 3 years, 10 males; Group 2). Platelet reactivity was assessed by flow cytometry as the increase in monocyte-platelet aggregates (MPA) and CD41 and PAC-1 platelet expression in response to exercise stress test (EST), adenosine diphosphate (ADP) stimulation (10 -7 M), or both. Endothelial function was assessed by measuring brachial artery dilation during post-ischaemic forearm hyperaemia [flow-mediated dilation (FMD)]. Both EST and ADP induced a higher percentage increase in platelet receptor expression in Group 1, compared with Group 2, with the most significant difference being shown for the response to the combined stimuli (e.g. MPA, 23.1 ± 12 vs. 5.63 ± 8, P <0.001; platelet PAC-1, 57.7 ± 47 vs. 13.2 ± 7, P <0.001). Compared with Group 2, Group 1 children showed lower FMD (10.7 ± 3.1 vs. 8.0 ± 2.9, respectively; P 0.007). However, no significant association was found between FMD and platelet reactivity. ConclusionOur results show increased platelet reactivity in children of patients with early AMI; the finding was not significantly correlated with endothelial dysfunction, suggesting that other mechanisms are mainly involved in the enhanced platelet response to agonistic stimuli.

AB - AimsTo assess whether platelet reactivity is increased in offspring of patients with early acute myocardial infarction (AMI) and its possible relation with endothelial dysfunction.Methods and resultsWe studied 23 healthy children (15 ± 3 years, 13 males) of patients with early AMI (≤50 years old; Group 1) and 21 healthy children of healthy subjects without any history of cardiovascular disease (14 ± 3 years, 10 males; Group 2). Platelet reactivity was assessed by flow cytometry as the increase in monocyte-platelet aggregates (MPA) and CD41 and PAC-1 platelet expression in response to exercise stress test (EST), adenosine diphosphate (ADP) stimulation (10 -7 M), or both. Endothelial function was assessed by measuring brachial artery dilation during post-ischaemic forearm hyperaemia [flow-mediated dilation (FMD)]. Both EST and ADP induced a higher percentage increase in platelet receptor expression in Group 1, compared with Group 2, with the most significant difference being shown for the response to the combined stimuli (e.g. MPA, 23.1 ± 12 vs. 5.63 ± 8, P <0.001; platelet PAC-1, 57.7 ± 47 vs. 13.2 ± 7, P <0.001). Compared with Group 2, Group 1 children showed lower FMD (10.7 ± 3.1 vs. 8.0 ± 2.9, respectively; P 0.007). However, no significant association was found between FMD and platelet reactivity. ConclusionOur results show increased platelet reactivity in children of patients with early AMI; the finding was not significantly correlated with endothelial dysfunction, suggesting that other mechanisms are mainly involved in the enhanced platelet response to agonistic stimuli.

KW - Endothelial dysfunction

KW - Family history of acute myocardial infarction

KW - Platelet aggregation

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