Platelet-Rich Plasma: The Choice of Activation Method Affects the Release of Bioactive Molecules.

Carola Cavallo, Alice Roffi, Brunella Grigolo, Erminia Mariani, Loredana Pratelli, Giulia Merli, Elizaveta Kon, Maurilio Marcacci, Giuseppe Filardo

Research output: Contribution to journalReview article

Abstract

Platelet-Rich Plasma (PRP) is a low-cost procedure to deliver high concentrations of autologous growth factors (GFs). Platelet activation is a crucial step that might influence the availability of bioactive molecules and therefore tissue healing. Activation of PRP from ten voluntary healthy males was performed by adding 10% of CaCl2, 10% of autologous thrombin, 10% of a mixture of CaCl2 + thrombin, and 10% of collagen type I. Blood derivatives were incubated for 15 and 30 minutes and 1, 2, and 24 hours and samples were evaluated for the release of VEGF, TGF-β1, PDGF-AB, IL-1β, and TNF-α. PRP activated with CaCl2, thrombin, and CaCl2/thrombin formed clots detected from the 15-minute evaluation, whereas in collagen-type-I-activated samples no clot formation was noticed. Collagen type I produced an overall lower GF release. Thrombin, CaCl2/thrombin, and collagen type I activated PRPs showed an immediate release of PDGF and TGF-β1 that remained stable over time, whereas VEGF showed an increasing trend from 15 minutes up to 24 hours. CaCl2 induced a progressive release of GFs from 15 minutes and increasing up to 24 hours. The method chosen to activate PRP influences both its physical form and the releasate in terms of GF amount and release kinetic.
Original languageEnglish
Number of pages8
JournalBioMed Research International
DOIs
Publication statusPublished - 2016

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Platelet-Rich Plasma
Platelets
Thrombin
Chemical activation
Collagen Type I
Plasmas
Molecules
Intercellular Signaling Peptides and Proteins
Vascular Endothelial Growth Factor A
Platelet Activation
Interleukin-1
Blood
Availability
Tissue
Derivatives
Costs and Cost Analysis
Kinetics
Costs

Cite this

@article{7e36f7e07b97486e8dfafe5dda3eef3d,
title = "Platelet-Rich Plasma: The Choice of Activation Method Affects the Release of Bioactive Molecules.",
abstract = "Platelet-Rich Plasma (PRP) is a low-cost procedure to deliver high concentrations of autologous growth factors (GFs). Platelet activation is a crucial step that might influence the availability of bioactive molecules and therefore tissue healing. Activation of PRP from ten voluntary healthy males was performed by adding 10{\%} of CaCl2, 10{\%} of autologous thrombin, 10{\%} of a mixture of CaCl2 + thrombin, and 10{\%} of collagen type I. Blood derivatives were incubated for 15 and 30 minutes and 1, 2, and 24 hours and samples were evaluated for the release of VEGF, TGF-β1, PDGF-AB, IL-1β, and TNF-α. PRP activated with CaCl2, thrombin, and CaCl2/thrombin formed clots detected from the 15-minute evaluation, whereas in collagen-type-I-activated samples no clot formation was noticed. Collagen type I produced an overall lower GF release. Thrombin, CaCl2/thrombin, and collagen type I activated PRPs showed an immediate release of PDGF and TGF-β1 that remained stable over time, whereas VEGF showed an increasing trend from 15 minutes up to 24 hours. CaCl2 induced a progressive release of GFs from 15 minutes and increasing up to 24 hours. The method chosen to activate PRP influences both its physical form and the releasate in terms of GF amount and release kinetic.",
author = "Carola Cavallo and Alice Roffi and Brunella Grigolo and Erminia Mariani and Loredana Pratelli and Giulia Merli and Elizaveta Kon and Maurilio Marcacci and Giuseppe Filardo",
year = "2016",
doi = "10.1155/2016/6591717",
language = "English",
journal = "BioMed Research International",
issn = "2314-6133",
publisher = "Hindawi Publishing Corporation",

}

TY - JOUR

T1 - Platelet-Rich Plasma: The Choice of Activation Method Affects the Release of Bioactive Molecules.

AU - Cavallo, Carola

AU - Roffi, Alice

AU - Grigolo, Brunella

AU - Mariani, Erminia

AU - Pratelli, Loredana

AU - Merli, Giulia

AU - Kon, Elizaveta

AU - Marcacci, Maurilio

AU - Filardo, Giuseppe

PY - 2016

Y1 - 2016

N2 - Platelet-Rich Plasma (PRP) is a low-cost procedure to deliver high concentrations of autologous growth factors (GFs). Platelet activation is a crucial step that might influence the availability of bioactive molecules and therefore tissue healing. Activation of PRP from ten voluntary healthy males was performed by adding 10% of CaCl2, 10% of autologous thrombin, 10% of a mixture of CaCl2 + thrombin, and 10% of collagen type I. Blood derivatives were incubated for 15 and 30 minutes and 1, 2, and 24 hours and samples were evaluated for the release of VEGF, TGF-β1, PDGF-AB, IL-1β, and TNF-α. PRP activated with CaCl2, thrombin, and CaCl2/thrombin formed clots detected from the 15-minute evaluation, whereas in collagen-type-I-activated samples no clot formation was noticed. Collagen type I produced an overall lower GF release. Thrombin, CaCl2/thrombin, and collagen type I activated PRPs showed an immediate release of PDGF and TGF-β1 that remained stable over time, whereas VEGF showed an increasing trend from 15 minutes up to 24 hours. CaCl2 induced a progressive release of GFs from 15 minutes and increasing up to 24 hours. The method chosen to activate PRP influences both its physical form and the releasate in terms of GF amount and release kinetic.

AB - Platelet-Rich Plasma (PRP) is a low-cost procedure to deliver high concentrations of autologous growth factors (GFs). Platelet activation is a crucial step that might influence the availability of bioactive molecules and therefore tissue healing. Activation of PRP from ten voluntary healthy males was performed by adding 10% of CaCl2, 10% of autologous thrombin, 10% of a mixture of CaCl2 + thrombin, and 10% of collagen type I. Blood derivatives were incubated for 15 and 30 minutes and 1, 2, and 24 hours and samples were evaluated for the release of VEGF, TGF-β1, PDGF-AB, IL-1β, and TNF-α. PRP activated with CaCl2, thrombin, and CaCl2/thrombin formed clots detected from the 15-minute evaluation, whereas in collagen-type-I-activated samples no clot formation was noticed. Collagen type I produced an overall lower GF release. Thrombin, CaCl2/thrombin, and collagen type I activated PRPs showed an immediate release of PDGF and TGF-β1 that remained stable over time, whereas VEGF showed an increasing trend from 15 minutes up to 24 hours. CaCl2 induced a progressive release of GFs from 15 minutes and increasing up to 24 hours. The method chosen to activate PRP influences both its physical form and the releasate in terms of GF amount and release kinetic.

U2 - 10.1155/2016/6591717

DO - 10.1155/2016/6591717

M3 - Review article

JO - BioMed Research International

JF - BioMed Research International

SN - 2314-6133

ER -