Platelets release their lysosomal content in vivo in humans upon activation

Silvia Ciferri, Carla Emiliani, Giuseppe Guglielmini, Antonio Orlacchio, Giuseppe G. Nenci, Paolo Gresele

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Platelets contain, besides α- and δ-granules, lysosomes which store glycohydrolases able to degrade glycoproteins, glycolipids and glycosaminoglycans. While several studies have shown that α- and δ-granule secretion takes place 'in vivo' in humans upon platelet activation, no data are available on the 'in vivo' release of lysosomes. We have studied the release of platelet lysosomal contents 'in vivo' in healthy volunteers at a localized site of platelet activation by measuring markers of lysosomal secretion in the blood oozing from a skin wound inflicted for the measurement of the bleeding-time. The levels of β-N-acetylhexosaminidase (Hex) were 13.1 ± 0.85 mU/ml in bleeding-time blood and 10.2 ± 0.66 mU/ml in plasma (p <0.001). Hex in serum was 16.4 ± 0.72 mU/ml. The levels of β-galactosidase were also higher in bleeding-time blood than in plasma (0.85 ± 0.07 mU/ml vs 0.4 ± 0.05 mU/ml, p <0.001). In bleeding-time blood collected at one minute intervals, Hex rose progressively consistent with ongoing platelet activation and flow-cytometry showed a progressive increase of the expression of LIMP and LAMP-2, two lysosomal associated proteins. In conclusion, our data demonstrate that platelet lysosomal glycohydrolases are released 'in vivo' in humans upon platelet activation.

Original languageEnglish
Pages (from-to)157-164
Number of pages8
JournalThrombosis and Haemostasis
Volume83
Issue number1
Publication statusPublished - 2000

Fingerprint

Bleeding Time
Platelet Activation
Blood Platelets
Glycoside Hydrolases
Lysosomes
Galactosidases
Glycolipids
Glycosaminoglycans
Glycoproteins
Healthy Volunteers
Flow Cytometry
Skin
Wounds and Injuries
Serum

Keywords

  • Bleeding time
  • Flow cytometry
  • LIMP and LAMP2
  • Platelet secretion
  • Platelet vessel wall interaction

ASJC Scopus subject areas

  • Hematology

Cite this

Ciferri, S., Emiliani, C., Guglielmini, G., Orlacchio, A., Nenci, G. G., & Gresele, P. (2000). Platelets release their lysosomal content in vivo in humans upon activation. Thrombosis and Haemostasis, 83(1), 157-164.

Platelets release their lysosomal content in vivo in humans upon activation. / Ciferri, Silvia; Emiliani, Carla; Guglielmini, Giuseppe; Orlacchio, Antonio; Nenci, Giuseppe G.; Gresele, Paolo.

In: Thrombosis and Haemostasis, Vol. 83, No. 1, 2000, p. 157-164.

Research output: Contribution to journalArticle

Ciferri, S, Emiliani, C, Guglielmini, G, Orlacchio, A, Nenci, GG & Gresele, P 2000, 'Platelets release their lysosomal content in vivo in humans upon activation', Thrombosis and Haemostasis, vol. 83, no. 1, pp. 157-164.
Ciferri S, Emiliani C, Guglielmini G, Orlacchio A, Nenci GG, Gresele P. Platelets release their lysosomal content in vivo in humans upon activation. Thrombosis and Haemostasis. 2000;83(1):157-164.
Ciferri, Silvia ; Emiliani, Carla ; Guglielmini, Giuseppe ; Orlacchio, Antonio ; Nenci, Giuseppe G. ; Gresele, Paolo. / Platelets release their lysosomal content in vivo in humans upon activation. In: Thrombosis and Haemostasis. 2000 ; Vol. 83, No. 1. pp. 157-164.
@article{af0cfe78af9f4779aa7c0eb43d4b46fa,
title = "Platelets release their lysosomal content in vivo in humans upon activation",
abstract = "Platelets contain, besides α- and δ-granules, lysosomes which store glycohydrolases able to degrade glycoproteins, glycolipids and glycosaminoglycans. While several studies have shown that α- and δ-granule secretion takes place 'in vivo' in humans upon platelet activation, no data are available on the 'in vivo' release of lysosomes. We have studied the release of platelet lysosomal contents 'in vivo' in healthy volunteers at a localized site of platelet activation by measuring markers of lysosomal secretion in the blood oozing from a skin wound inflicted for the measurement of the bleeding-time. The levels of β-N-acetylhexosaminidase (Hex) were 13.1 ± 0.85 mU/ml in bleeding-time blood and 10.2 ± 0.66 mU/ml in plasma (p <0.001). Hex in serum was 16.4 ± 0.72 mU/ml. The levels of β-galactosidase were also higher in bleeding-time blood than in plasma (0.85 ± 0.07 mU/ml vs 0.4 ± 0.05 mU/ml, p <0.001). In bleeding-time blood collected at one minute intervals, Hex rose progressively consistent with ongoing platelet activation and flow-cytometry showed a progressive increase of the expression of LIMP and LAMP-2, two lysosomal associated proteins. In conclusion, our data demonstrate that platelet lysosomal glycohydrolases are released 'in vivo' in humans upon platelet activation.",
keywords = "Bleeding time, Flow cytometry, LIMP and LAMP2, Platelet secretion, Platelet vessel wall interaction",
author = "Silvia Ciferri and Carla Emiliani and Giuseppe Guglielmini and Antonio Orlacchio and Nenci, {Giuseppe G.} and Paolo Gresele",
year = "2000",
language = "English",
volume = "83",
pages = "157--164",
journal = "Thrombosis and Haemostasis",
issn = "0340-6245",
publisher = "Schattauer GmbH",
number = "1",

}

TY - JOUR

T1 - Platelets release their lysosomal content in vivo in humans upon activation

AU - Ciferri, Silvia

AU - Emiliani, Carla

AU - Guglielmini, Giuseppe

AU - Orlacchio, Antonio

AU - Nenci, Giuseppe G.

AU - Gresele, Paolo

PY - 2000

Y1 - 2000

N2 - Platelets contain, besides α- and δ-granules, lysosomes which store glycohydrolases able to degrade glycoproteins, glycolipids and glycosaminoglycans. While several studies have shown that α- and δ-granule secretion takes place 'in vivo' in humans upon platelet activation, no data are available on the 'in vivo' release of lysosomes. We have studied the release of platelet lysosomal contents 'in vivo' in healthy volunteers at a localized site of platelet activation by measuring markers of lysosomal secretion in the blood oozing from a skin wound inflicted for the measurement of the bleeding-time. The levels of β-N-acetylhexosaminidase (Hex) were 13.1 ± 0.85 mU/ml in bleeding-time blood and 10.2 ± 0.66 mU/ml in plasma (p <0.001). Hex in serum was 16.4 ± 0.72 mU/ml. The levels of β-galactosidase were also higher in bleeding-time blood than in plasma (0.85 ± 0.07 mU/ml vs 0.4 ± 0.05 mU/ml, p <0.001). In bleeding-time blood collected at one minute intervals, Hex rose progressively consistent with ongoing platelet activation and flow-cytometry showed a progressive increase of the expression of LIMP and LAMP-2, two lysosomal associated proteins. In conclusion, our data demonstrate that platelet lysosomal glycohydrolases are released 'in vivo' in humans upon platelet activation.

AB - Platelets contain, besides α- and δ-granules, lysosomes which store glycohydrolases able to degrade glycoproteins, glycolipids and glycosaminoglycans. While several studies have shown that α- and δ-granule secretion takes place 'in vivo' in humans upon platelet activation, no data are available on the 'in vivo' release of lysosomes. We have studied the release of platelet lysosomal contents 'in vivo' in healthy volunteers at a localized site of platelet activation by measuring markers of lysosomal secretion in the blood oozing from a skin wound inflicted for the measurement of the bleeding-time. The levels of β-N-acetylhexosaminidase (Hex) were 13.1 ± 0.85 mU/ml in bleeding-time blood and 10.2 ± 0.66 mU/ml in plasma (p <0.001). Hex in serum was 16.4 ± 0.72 mU/ml. The levels of β-galactosidase were also higher in bleeding-time blood than in plasma (0.85 ± 0.07 mU/ml vs 0.4 ± 0.05 mU/ml, p <0.001). In bleeding-time blood collected at one minute intervals, Hex rose progressively consistent with ongoing platelet activation and flow-cytometry showed a progressive increase of the expression of LIMP and LAMP-2, two lysosomal associated proteins. In conclusion, our data demonstrate that platelet lysosomal glycohydrolases are released 'in vivo' in humans upon platelet activation.

KW - Bleeding time

KW - Flow cytometry

KW - LIMP and LAMP2

KW - Platelet secretion

KW - Platelet vessel wall interaction

UR - http://www.scopus.com/inward/record.url?scp=0033964465&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033964465&partnerID=8YFLogxK

M3 - Article

C2 - 10669170

AN - SCOPUS:0033964465

VL - 83

SP - 157

EP - 164

JO - Thrombosis and Haemostasis

JF - Thrombosis and Haemostasis

SN - 0340-6245

IS - 1

ER -