Ploidy and proliferative activity of human brain tumors. A flow cytofluorometric study

M. Danova, A. Riccardi, G. Mazzini, G. Ucci, P. Gaetani, V. Silvani, R. Knerich, G. Butti, E. Ascari

Research output: Contribution to journalArticlepeer-review

Abstract

Ploidy and proliferative characteristics were estimated by flow cytometry of the nuclear DNA content of 92 human brain tumors. Samples were frozen at -20°C immediately after surgery and single cell suspensions were obtained with a mechanical dissociation technique. Propidium iodide was employed for nuclear DNA staining. Human normal brain tissue was used as internal diploid reference standard. 86% of benign tumors had unimodal DNA distribution with a DNA index (DNA I = modal channel of the G(0/1) peak of the studied population/modal channel of the G(0/1) peak of the normal brain) usually within the diploid or near-diploid range. 14.0% had aneuploidy, with an additional cell peak having a median DNA I of 1.60. Among malignant tumors, these figures were 61.2 and 38.8% (p <0.001). The percentage of S phase cells was higher in malignant (median = 3.6) than in benign tumors (median = 2.0, p <0.01), without correlation to histological tumor subtype. Flow cytometry appears to be a useful method for evaluating differences in DNA distribution in tumors of the central nervous system.

Original languageEnglish
Pages (from-to)102-107
Number of pages6
JournalOncology
Volume44
Issue number2
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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