Ploidy and proliferative activity of human gastric carcinoma: A cytofluorometric study on fresh and on paraffin embedded material

M. Danova, G. Mazzini, G. Wilson, G. Ucci, P. Dionigi, A. Riccardi, R. Fiocca

Research output: Contribution to journalArticle

Abstract

Flow cytometric DNA analysis was performed on both fresh and on paraffin embedded samples obtained by gastroscopic biopsies in 5 patients with histologically normal gastric mucosa (20 specimens) and by radical gastrectomies in 9 cases of human gastric cancer (36 specimens). Ploidy and the distribution of cells in the different cell cycle phases were estimated. Results from fresh tissue were compared with those from paraffin embedded material. All the samples of normal mucosa showed a diploid modal DNA content. Unimodal DNA distribution was found both in fresh and paraffin embedded material of 3 well differentiated gastric adenocarcinomas, whereas the remaining 6 tumors (1 moderately differentiated, 4 poorly differentiated and 1 undifferentiated adenocarcinomas) showed aneuploidy. The percentage of cells in S phase in normal tissue and in tumors were respectively 9.0% and 11.9% in the fresh material, and 10.0% and 15.0% in the paraffin embedded material. No statistically significant differences were found between fresh and paraffin embedded samples, whereas the proliferative activity was, in both cases, statistically higher in tumors than in normal mucosa (p

Original languageEnglish
Pages (from-to)73-82
Number of pages10
JournalBasic and Applied Histochemistry
Volume31
Issue number1
Publication statusPublished - 1987

ASJC Scopus subject areas

  • Anatomy

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