Poised transcription factories prime silent uPA gene prior to activation

Carmelo Ferrai, Sheila Q. Xie, Paolo Luraghi, Davide Munari, Francisco Ramirez, Miguel R. Branco, Ana Pombo, Massimo P. Crippa

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The position of genes in the interphase nucleus and their association with functional landmarks correlate with active and/or silent states of expression. Gene activation can induce chromatin looping from chromosome territories (CTs) and is thought to require de novo association with transcription factories. We identify two types of factory: "poised transcription factories," containing RNA polymerase II phosphorylated on Ser5, but not Ser2, residues, which differ from "active factories" associated with phosphorylation on both residues. Using the urokinase-type plasminogen activator (uPA) gene as a model system, we find that this inducible gene is predominantly associated with poised (S5p+S2p-) factories prior to activation and localized at the CT interior. Shortly after induction, the uPA locus is found associated with active (S5p+S2p+) factories and loops out from its CT. However, the levels of gene association with poised or active transcription factories, before and after activation, are independent of locus positioning relative to its CT. RNA-FISH analyses show that, after activation, the uPA gene is transcribed with the same frequency at each CT position. Unexpectedly, prior to activation, the uPA loci internal to the CT are seldom transcriptionally active, while the smaller number of uPA loci found outside their CT are transcribed as frequently as after induction. The association of inducible genes with poised transcription factories prior to activation is likely to contribute to the rapid and robust induction of gene expression in response to external stimuli, whereas gene positioning at the CT interior may be important to reinforce silencing mechanisms prior to induction.

Original languageEnglish
Article numbere1000270
JournalPLoS Biology
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 2010

Fingerprint

u-plasminogen activator
Urokinase-Type Plasminogen Activator
Transcription
Chromosomes
factories
Industrial plants
transcription (genetics)
Genes
Chemical activation
chromosomes
genes
Association reactions
loci
Chromosome Positioning
Gene Order
RNA Polymerase II
Interphase
Phosphorylation
gene activation
Transcriptional Activation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Ferrai, C., Xie, S. Q., Luraghi, P., Munari, D., Ramirez, F., Branco, M. R., ... Crippa, M. P. (2010). Poised transcription factories prime silent uPA gene prior to activation. PLoS Biology, 8(1), [e1000270]. https://doi.org/10.1371/journal.pbio.1000270

Poised transcription factories prime silent uPA gene prior to activation. / Ferrai, Carmelo; Xie, Sheila Q.; Luraghi, Paolo; Munari, Davide; Ramirez, Francisco; Branco, Miguel R.; Pombo, Ana; Crippa, Massimo P.

In: PLoS Biology, Vol. 8, No. 1, e1000270, 01.2010.

Research output: Contribution to journalArticle

Ferrai, C, Xie, SQ, Luraghi, P, Munari, D, Ramirez, F, Branco, MR, Pombo, A & Crippa, MP 2010, 'Poised transcription factories prime silent uPA gene prior to activation', PLoS Biology, vol. 8, no. 1, e1000270. https://doi.org/10.1371/journal.pbio.1000270
Ferrai C, Xie SQ, Luraghi P, Munari D, Ramirez F, Branco MR et al. Poised transcription factories prime silent uPA gene prior to activation. PLoS Biology. 2010 Jan;8(1). e1000270. https://doi.org/10.1371/journal.pbio.1000270
Ferrai, Carmelo ; Xie, Sheila Q. ; Luraghi, Paolo ; Munari, Davide ; Ramirez, Francisco ; Branco, Miguel R. ; Pombo, Ana ; Crippa, Massimo P. / Poised transcription factories prime silent uPA gene prior to activation. In: PLoS Biology. 2010 ; Vol. 8, No. 1.
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