TY - JOUR
T1 - Poloxamer 338 affects cell adhesion and biofilm formation in escherichia coli
T2 - Potential applications in the management of catheter-associated urinary tract infections
AU - Stirpe, Mariarita
AU - Brugnoli, Benedetta
AU - Donelli, Gianfranco
AU - Francolini, Iolanda
AU - Vuotto, Claudia
N1 - Funding Information:
Partial fundings for this study have been obtained by Gianfranco Donelli and Claudia Vuotto from Hutchison Biofilm Medical Solutions Limited, of Hutchison House, 5 Hester Road, Battersea, London, SWll 4AN, United Kingdom, and by Iolanda Francolini from Sapienza University of Rome (Project n. RP11715C785E4434). Biological samples for this study were obtained from the Biobank of the Fondazione Santa Lucia, Rome-Italy.
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/11
Y1 - 2020/11
N2 - Poloxamers are nontoxic, amphiphilic copolymers used in different formulations. Due to its surfactant properties, Poloxamer 338 (P388) is herein proposed as a strategy to avoid biofilm formation often causing recalcitrant catheter-associated urinary tract infections (CAUTI). The aim is to evaluate the ability of P388 coatings to affect the adhesion of Ec5FSL and Ec9FSL Escherichia coli strains on silicone urinary catheters. Attenuated total reflection infrared spectroscopy, atomic force microscopy, and static water contact angle measurement were employed to characterize the P388-coated silicone catheter in terms of amount of P388 layered, coating thickness, homogeneity, and hydrophilicity. In static conditions, the antifouling power of P388 was defined by comparing the E. coli cells adherent on a hydrophilic P388-adsorbed catheter segment with those on an uncoated one. A P388-coated catheter, having a homogeneous coverage of 35 nm in thickness, reduced of 0.83 log10 and 0.51 log10 the biofilm of Ec5FSL and Ec9FSL, respectively. In dynamic conditions, the percentage of cell adhesion on P388-adsorbed silicone channels was investigated by a microfluidic system, simulating the in vivo conditions of catheterized patients. As a result, both E. coli isolates were undetected. The strong and stable antifouling property against E. coli biofilm lead us to consider P388 as a promising anti-biofilm agent for CAUTIs control.
AB - Poloxamers are nontoxic, amphiphilic copolymers used in different formulations. Due to its surfactant properties, Poloxamer 338 (P388) is herein proposed as a strategy to avoid biofilm formation often causing recalcitrant catheter-associated urinary tract infections (CAUTI). The aim is to evaluate the ability of P388 coatings to affect the adhesion of Ec5FSL and Ec9FSL Escherichia coli strains on silicone urinary catheters. Attenuated total reflection infrared spectroscopy, atomic force microscopy, and static water contact angle measurement were employed to characterize the P388-coated silicone catheter in terms of amount of P388 layered, coating thickness, homogeneity, and hydrophilicity. In static conditions, the antifouling power of P388 was defined by comparing the E. coli cells adherent on a hydrophilic P388-adsorbed catheter segment with those on an uncoated one. A P388-coated catheter, having a homogeneous coverage of 35 nm in thickness, reduced of 0.83 log10 and 0.51 log10 the biofilm of Ec5FSL and Ec9FSL, respectively. In dynamic conditions, the percentage of cell adhesion on P388-adsorbed silicone channels was investigated by a microfluidic system, simulating the in vivo conditions of catheterized patients. As a result, both E. coli isolates were undetected. The strong and stable antifouling property against E. coli biofilm lead us to consider P388 as a promising anti-biofilm agent for CAUTIs control.
KW - Antifouling coatings
KW - Biofilm
KW - Escherichia coli
KW - Poloxamer 338
KW - Urinary catheter
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U2 - 10.3390/pathogens9110885
DO - 10.3390/pathogens9110885
M3 - Article
AN - SCOPUS:85094154056
VL - 9
SP - 1
EP - 16
JO - Pathogens
JF - Pathogens
SN - 2076-0817
IS - 11
M1 - 885
ER -