TY - JOUR
T1 - Poly(ε-caprolactone) reinforced with sol-gel synthesized organic-inorganic hybrid fillers as composite substrates for tissue engineering
AU - Russo, Teresa
AU - Gloria, Antonio
AU - D'Antò, Vincenzo
AU - D'Amora, Ugo
AU - Ametrano, Gianluca
AU - Bollino, Flavia
AU - De Santis, Roberto
AU - Ausanio, Giovanni
AU - Catauro, Michelina
AU - Rengo, Sandro
AU - Ambrosio, Luigi
PY - 2010/9
Y1 - 2010/9
N2 - Purpose: The importance of polymer-based composite materials to make multifunctional substrates for tissue engineering and the strategies to improve their performances have been stressed in the literature. Bioactive features of sol-gel synthesized poly(ε-caprolactone)/TiO2 or poly(ε-caprolactone)/ZrO2 organic-inorganic hybrid materials are widely documented. Accordingly, the aim of this preliminary research was to develop advanced composite substrates consisting of a poly(ε-caprolactone) matrix reinforced with sol-gel synthesized PCL/TiO2 or PCL/ZrO 2 hybrid fillers. Methods: Micro-computed tomography and atomic force microscopy analyses allowed to study surface topography and roughness. On the other hand, mechanical and biological performances were evaluated by small punch tests and Alamar Blue™ assay, respectively. Results: Micro-computed tomography and atomic force microscopy analyses highlighted the effect of the preparation technique. Results from small punch tests and Alamar Blue™ assay evidenced that PCL reinforced with Ti2 (PCL=12, TiO2=88 wt%) and Zr2 (PCL=12, ZrO2=88 wt%) hybrid fillers provided better mechanical and biological performances. Conclusions: PCL reinforced with Ti2 (PCL=12, TiO2=88 wt%) and Zr2 (PCL=12, ZrO2=88 wt%) hybrid fillers could be considered as advanced composite substrates for hard tissue engineering.
AB - Purpose: The importance of polymer-based composite materials to make multifunctional substrates for tissue engineering and the strategies to improve their performances have been stressed in the literature. Bioactive features of sol-gel synthesized poly(ε-caprolactone)/TiO2 or poly(ε-caprolactone)/ZrO2 organic-inorganic hybrid materials are widely documented. Accordingly, the aim of this preliminary research was to develop advanced composite substrates consisting of a poly(ε-caprolactone) matrix reinforced with sol-gel synthesized PCL/TiO2 or PCL/ZrO 2 hybrid fillers. Methods: Micro-computed tomography and atomic force microscopy analyses allowed to study surface topography and roughness. On the other hand, mechanical and biological performances were evaluated by small punch tests and Alamar Blue™ assay, respectively. Results: Micro-computed tomography and atomic force microscopy analyses highlighted the effect of the preparation technique. Results from small punch tests and Alamar Blue™ assay evidenced that PCL reinforced with Ti2 (PCL=12, TiO2=88 wt%) and Zr2 (PCL=12, ZrO2=88 wt%) hybrid fillers provided better mechanical and biological performances. Conclusions: PCL reinforced with Ti2 (PCL=12, TiO2=88 wt%) and Zr2 (PCL=12, ZrO2=88 wt%) hybrid fillers could be considered as advanced composite substrates for hard tissue engineering.
KW - Alamar Blue™
KW - Assay
KW - Atomic force microscopy
KW - Composite substrate
KW - Organic-inorganic hybrid
KW - Poly(ε-caprolactone)
KW - Small punch test
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U2 - 10.5301/JABB.2010.6094
DO - 10.5301/JABB.2010.6094
M3 - Article
C2 - 21337305
AN - SCOPUS:78951470550
VL - 8
SP - 146
EP - 152
JO - Journal of Applied Biomaterials and Biomechanics
JF - Journal of Applied Biomaterials and Biomechanics
SN - 1722-6899
IS - 3
ER -