Abstract
Poly(ADP-ribosylation) is a post-translational modification of proteins playing a crucial role in many processes, including DNA repair and cell death. The best known poly(ADP-ribosylating) enzime, PARP-1, is a DNA nick sensor and uses βNAD+ to form polymers of ADP-ribose which are further arefurther bound to nuclear protein acceptors. To strictly regulate poly(ADP-ribose) turnover, its degradation is assured by the enzyme poly(ADP-ribose) glycohydrolase (PARG). During apoptosis, PARP-1 plays two opposite roles: its stimulation leads to poly(ADP-ribose) synthesis, whereas caspases cause PARP-1 cleavage and inactivation. PARP-1 proteolysis produces an 89 kDa C-terminal fragment, with a reduced catalytic activity, and a 24 kDa N-terminal peptide, which retains the DNA binding domains. The fate and the possible role of these fragments during apoptosis will be discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 321-328 |
| Number of pages | 8 |
| Journal | Apoptosis |
| Volume | 7 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2002 |
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Keywords
- Apoptosis
- Autoimmunity
- Caspases
- PARP-1
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
Cite this
Poly(ADP-ribose) polymerase-1 cleavage during apoptosis : An update. / Soldani, C.; Scovassi, A. I.
In: Apoptosis, Vol. 7, No. 4, 2002, p. 321-328.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Poly(ADP-ribose) polymerase-1 cleavage during apoptosis
T2 - An update
AU - Soldani, C.
AU - Scovassi, A. I.
PY - 2002
Y1 - 2002
N2 - Poly(ADP-ribosylation) is a post-translational modification of proteins playing a crucial role in many processes, including DNA repair and cell death. The best known poly(ADP-ribosylating) enzime, PARP-1, is a DNA nick sensor and uses βNAD+ to form polymers of ADP-ribose which are further arefurther bound to nuclear protein acceptors. To strictly regulate poly(ADP-ribose) turnover, its degradation is assured by the enzyme poly(ADP-ribose) glycohydrolase (PARG). During apoptosis, PARP-1 plays two opposite roles: its stimulation leads to poly(ADP-ribose) synthesis, whereas caspases cause PARP-1 cleavage and inactivation. PARP-1 proteolysis produces an 89 kDa C-terminal fragment, with a reduced catalytic activity, and a 24 kDa N-terminal peptide, which retains the DNA binding domains. The fate and the possible role of these fragments during apoptosis will be discussed.
AB - Poly(ADP-ribosylation) is a post-translational modification of proteins playing a crucial role in many processes, including DNA repair and cell death. The best known poly(ADP-ribosylating) enzime, PARP-1, is a DNA nick sensor and uses βNAD+ to form polymers of ADP-ribose which are further arefurther bound to nuclear protein acceptors. To strictly regulate poly(ADP-ribose) turnover, its degradation is assured by the enzyme poly(ADP-ribose) glycohydrolase (PARG). During apoptosis, PARP-1 plays two opposite roles: its stimulation leads to poly(ADP-ribose) synthesis, whereas caspases cause PARP-1 cleavage and inactivation. PARP-1 proteolysis produces an 89 kDa C-terminal fragment, with a reduced catalytic activity, and a 24 kDa N-terminal peptide, which retains the DNA binding domains. The fate and the possible role of these fragments during apoptosis will be discussed.
KW - Apoptosis
KW - Autoimmunity
KW - Caspases
KW - PARP-1
UR - http://www.scopus.com/inward/record.url?scp=0036022402&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036022402&partnerID=8YFLogxK
U2 - 10.1023/A:1016119328968
DO - 10.1023/A:1016119328968
M3 - Article
VL - 7
SP - 321
EP - 328
JO - Apoptosis : an international journal on programmed cell death
JF - Apoptosis : an international journal on programmed cell death
SN - 1360-8185
IS - 4
ER -