Poly(ADP-ribose) polymerase cleavage during apoptosis: When and where?

Cristiana Soldani, Maria Claudia Lazzè, Maria Grazia Bottone, Gianluca Tognon, Marco Biggiogera, Carlo E. Pellicciari, A. Ivana Scovassi

Research output: Contribution to journalArticlepeer-review

Abstract

Poly(ADP-ribose) polymerase-1 (PARP-1) plays the active role of "nick sensor" during DNA repair and apoptosis, when it synthesizes ADP-ribose from NAD+ in the presence of DNA strand breaks. Moreover, PARP-1 becomes a target of apoptotic caspases, which originate two proteolytic fragments of 89 and 24 kDa. The precise relationship between PARP-1 activation and degradation during apoptosis is still a matter of debate. In human Hep-2 cells driven to apoptosis by actinomycin D, we have monitored PARP-1 activity by the mAb 10H, which is specific for the ADP-ribose polymers, and we have observed that poly(ADP-ribose) synthesis is a very early response to the apoptotic stimulus. The analysis of the presence and fate of the p89 proteolytic fragment revealed that PARP-1 proteolysis by caspases is concomitant with poly(ADP-ribose) synthesis and that p89 migrates from the nucleus into the cytoplasm in late apoptotic cells with advanced nuclear fragmentation.

Original languageEnglish
Pages (from-to)193-201
Number of pages9
JournalExperimental Cell Research
Volume269
Issue number2
DOIs
Publication statusPublished - Oct 1 2001

Keywords

  • Actinomycin D
  • Apoptosis
  • Caspases
  • PARP-1
  • Poly(ADP-ribose)

ASJC Scopus subject areas

  • Cell Biology

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