Poly(amido-amine)-based hydrogels with tailored mechanical properties and degradation rates for tissue engineering

Federico Martello, Alessandro Tocchio, Margherita Tamplenizza, Irini Gerges, Valentina Pistis, Rossella Recenti, Monica Bortolin, Massimo Del Fabbro, Simona Argentiere, Paolo Milani, Cristina Lenardi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Poly(amido-amine) (PAA) hydrogels containing the 2,2-bisacrylamidoacetic acid-4-amminobutyl guanidine monomeric unit have a known ability to enhance cellular adhesion by interacting with the arginin-glycin-aspartic acid (RGD)-binding αVβ3 integrin, expressed by a wide number of cell types. Scientific interest in this class of materials has traditionally been hampered by their poor mechanical properties and restricted range of degradation rate. Here we present the design of novel biocompatible, RGD-mimic PAA-based hydrogels with wide and tunable degradation rates as well as improved mechanical and biological properties for biomedical applications. This is achieved by radical polymerization of acrylamide-terminated PAA oligomers in both the presence and absence of 2-hydroxyethylmethacrylate. The degradation rate is found to be precisely tunable by adjusting the PAA oligomer molecular weight and acrylic co-monomer concentration in the starting reaction mixture. Cell adhesion and proliferation tests on Madin-Darby canine kidney epithelial cells show that PAA-based hydrogels have the capacity to promote cell adhesion up to 200% compared to the control. Mechanical tests show higher compressive strength of acrylic chain containing hydrogels compared to traditional PAA hydrogels.

Original languageEnglish
Pages (from-to)1206-1215
Number of pages10
JournalActa Biomaterialia
Volume10
Issue number3
DOIs
Publication statusPublished - Mar 2014

Fingerprint

Hydrogels
Tissue Engineering
Tissue engineering
Amines
Degradation
Mechanical properties
Cell adhesion
Oligomers
Cell Adhesion
Acrylics
Compressive Strength
Madin Darby Canine Kidney Cells
Acids
Acrylamide
Guanidine
Cell proliferation
Free radical polymerization
Aspartic Acid
Integrins
Polymerization

Keywords

  • Biomimetics
  • Hydrogels
  • Structure-property relationship
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Medicine(all)

Cite this

Martello, F., Tocchio, A., Tamplenizza, M., Gerges, I., Pistis, V., Recenti, R., ... Lenardi, C. (2014). Poly(amido-amine)-based hydrogels with tailored mechanical properties and degradation rates for tissue engineering. Acta Biomaterialia, 10(3), 1206-1215. https://doi.org/10.1016/j.actbio.2013.12.023

Poly(amido-amine)-based hydrogels with tailored mechanical properties and degradation rates for tissue engineering. / Martello, Federico; Tocchio, Alessandro; Tamplenizza, Margherita; Gerges, Irini; Pistis, Valentina; Recenti, Rossella; Bortolin, Monica; Del Fabbro, Massimo; Argentiere, Simona; Milani, Paolo; Lenardi, Cristina.

In: Acta Biomaterialia, Vol. 10, No. 3, 03.2014, p. 1206-1215.

Research output: Contribution to journalArticle

Martello, F, Tocchio, A, Tamplenizza, M, Gerges, I, Pistis, V, Recenti, R, Bortolin, M, Del Fabbro, M, Argentiere, S, Milani, P & Lenardi, C 2014, 'Poly(amido-amine)-based hydrogels with tailored mechanical properties and degradation rates for tissue engineering', Acta Biomaterialia, vol. 10, no. 3, pp. 1206-1215. https://doi.org/10.1016/j.actbio.2013.12.023
Martello, Federico ; Tocchio, Alessandro ; Tamplenizza, Margherita ; Gerges, Irini ; Pistis, Valentina ; Recenti, Rossella ; Bortolin, Monica ; Del Fabbro, Massimo ; Argentiere, Simona ; Milani, Paolo ; Lenardi, Cristina. / Poly(amido-amine)-based hydrogels with tailored mechanical properties and degradation rates for tissue engineering. In: Acta Biomaterialia. 2014 ; Vol. 10, No. 3. pp. 1206-1215.
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