Polyamine depletion protects HL-60 cells from 2-deoxy-D-ribose-induced apoptosis

Maria Giuseppina Monti, Stefania Ghiaroni, Laura Pernecco, Daniela Barbieri, Gaetano Marverti, Claudio Franceschi

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated the involvement of natural polyamines in HL-60 cell death triggered by exposure to 2-deoxy-D-ribose (dRib). In contrast to previous studies, exogenous polyamines failed to protect HL-60 cells against apoptosis caused by dRib. Moreover, in our experimental conditions, depletion of intracellular levels of putrescine and spermidine by α-difluoromethylornithine (DFMO) delayed the onset of apoptosis by at least a day or so. Exogenous polyamines reversed the beneficial effect of DFMO and restored the apoptotic levels observed in dRib-treated cells. We suggested that polyamines, especially putrescine and spermidine, act as facilitating factors in the induction of apoptosis triggered by dRib in HL-60 cells.

Original languageEnglish
Pages (from-to)799-806
Number of pages8
JournalLife Sciences
Volume62
Issue number9
DOIs
Publication statusPublished - Jan 23 1998

Keywords

  • 2-deoxy-D-ribose
  • Apoptosis
  • HL-60 cells
  • Polyamines

ASJC Scopus subject areas

  • Pharmacology

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