Polychlorinated biphenyls induce mitochondrial dysfunction in SH-SY5Y neuroblastoma cells

Stefania Cocco, Agnese Secondo, Adelaide Del Viscovo, Claudio Procaccini, Luigi Formisano, Cristina Franco, Alba Esposito, Antonella Scorziello, Giuseppe Matarese, Gianfranco Di Renzo, Lorella Maria Teresa Canzoniero

Research output: Contribution to journalArticle

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Abstract

Chronic exposure to polychlorinated biphenyls (PCBs), ubiquitous environmental contaminants, can adversely affect the development and function of the nervous system. Here we evaluated the effect of PCB exposure on mitochondrial function using the PCB mixture Aroclor-1254 (A1254) in SH-SY5Y neuroblastoma cells. A 6-hour exposure to A1254 (5 μg/ml) reduced cellular ATP production by 45%±7, and mitochondrial membrane potential, detected by TMRE, by 49%±7. Consistently, A1254 significantly decreased oxidative phosphorylation and aerobic glycolysis measured by extracellular flux analyzer. Furthermore, the activity of mitochondrial protein complexes I, II, and IV, but not V (ATPase), measured by BN-PAGE technique, was significantly reduced after 6-hour exposure to A1254. The addition of pyruvic acid during exposure to A1254 significantly prevent A1254-induced cell injury, restoring resting mitochondrial membrane potential, ATP levels, oxidative phosphorylation and aerobic glycolysis. Furthermore, pyruvic acid significantly preserved the activity of mitochondrial complexes I, II and IV and increased basal activity of complex V. Collectively, the present results indicate that the neurotoxicity of A1254 depends on the impairment of oxidative phosphorylation, aerobic glycolysis, and mitochondrial complexes I, II, and IV activity and it was counteracted by pyruvic acid.

Original languageEnglish
Article numbere0129481
JournalPLoS One
Volume10
Issue number6
DOIs
Publication statusPublished - Jun 23 2015

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Chlorodiphenyl (54% Chlorine)
NADH dehydrogenase (ubiquinone)
pyruvic acid
oxidative phosphorylation
Polychlorinated Biphenyls
polychlorinated biphenyls
glycolysis
Neuroblastoma
membrane potential
Oxidative Phosphorylation
Glycolysis
Pyruvic Acid
H-transporting ATP synthase
neurotoxicity
cells
chronic exposure
Mitochondrial Membrane Potential
nervous system
pollution
Adenosine Triphosphate

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Cocco, S., Secondo, A., Del Viscovo, A., Procaccini, C., Formisano, L., Franco, C., ... Canzoniero, L. M. T. (2015). Polychlorinated biphenyls induce mitochondrial dysfunction in SH-SY5Y neuroblastoma cells. PLoS One, 10(6), [e0129481]. https://doi.org/10.1371/journal.pone.0129481

Polychlorinated biphenyls induce mitochondrial dysfunction in SH-SY5Y neuroblastoma cells. / Cocco, Stefania; Secondo, Agnese; Del Viscovo, Adelaide; Procaccini, Claudio; Formisano, Luigi; Franco, Cristina; Esposito, Alba; Scorziello, Antonella; Matarese, Giuseppe; Di Renzo, Gianfranco; Canzoniero, Lorella Maria Teresa.

In: PLoS One, Vol. 10, No. 6, e0129481, 23.06.2015.

Research output: Contribution to journalArticle

Cocco, S, Secondo, A, Del Viscovo, A, Procaccini, C, Formisano, L, Franco, C, Esposito, A, Scorziello, A, Matarese, G, Di Renzo, G & Canzoniero, LMT 2015, 'Polychlorinated biphenyls induce mitochondrial dysfunction in SH-SY5Y neuroblastoma cells', PLoS One, vol. 10, no. 6, e0129481. https://doi.org/10.1371/journal.pone.0129481
Cocco S, Secondo A, Del Viscovo A, Procaccini C, Formisano L, Franco C et al. Polychlorinated biphenyls induce mitochondrial dysfunction in SH-SY5Y neuroblastoma cells. PLoS One. 2015 Jun 23;10(6). e0129481. https://doi.org/10.1371/journal.pone.0129481
Cocco, Stefania ; Secondo, Agnese ; Del Viscovo, Adelaide ; Procaccini, Claudio ; Formisano, Luigi ; Franco, Cristina ; Esposito, Alba ; Scorziello, Antonella ; Matarese, Giuseppe ; Di Renzo, Gianfranco ; Canzoniero, Lorella Maria Teresa. / Polychlorinated biphenyls induce mitochondrial dysfunction in SH-SY5Y neuroblastoma cells. In: PLoS One. 2015 ; Vol. 10, No. 6.
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