Polyclonal activation of human B lymphocytes in vitro by pokeweed mitogen: A simple technique for the simultaneous assessment of cell proliferation, generation of plasma cells, plaque-forming cells and immunoglobulin production

L. Nespoli, A. Vitiello, R. Maccario, A. Lanzavecchia, A. G. Ugazio

Research output: Contribution to journalArticle

Abstract

A simple technique has been worked out for the simultaneous assessment of cell proliferation, generation of plasma cells, plaque-forming cells and immunoglobulin production from single cultures of 1 x 10 6 human peripheral blood lymphocytes stimulated with pokeweed mitogen. Kinetic studies showed that number of cells, thymidine incorporation rate, number of plaque-forming cells against fluorescein-isothiocyanate-haptenated sheep erythrocytes and concentration of IgM and IgG in the supernatant peaked on the average between day 5 and 7 of culture. This technique is particularly suitable for the analysis of hypogammaglobulinaemias in infancy.

Original languageEnglish
Pages (from-to)489-496
Number of pages8
JournalScandinavian Journal of Immunology
Volume8
Issue number6
Publication statusPublished - 1978

ASJC Scopus subject areas

  • Immunology

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