Polyclonal B lymphocyte activation in progressive systemic sclerosis

G. Famularo, R. Giacomelli, E. Alesse, M. G. Cifone, S. Morrone, M. Boirivant, C. Danese, M. A. Perego, A. Santoni, G. Tonietti

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Abstract

In 13 women affected by progressive systemic sclerosis (PSS) the activation state of circulating B lymphocytes by use of monoclonal antibodies and the in vitro IgG, IgA, IgM synthesis by peripheral blood mononuclear cells (PBMC) cultured with or without pokeweed-mitogen (PWM) were evaluated. The total and ''activated'' B lymphocytes were increased. The supernatants of PBMC cultured without PWM contained increased IgG and IgA levels; only in two patients, however, were IgM levels increased. The addition of PWM augmented IgG and IgA levels only in three cases and never IgM levels. Our results indicate that the polyclonal hyperactivity of B lymphocytes is a major immunologic feature in PSS patients. The lack of increase of in vitro immunoglobulin synthesis in the presence of PWM suggests either that B lymphocytes, already maximally activated in vivo, are unresponsive to further mitogen stimulation or that a more complex imbalance of B lymphocyte responsiveness to PWM may exist.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalJournal of Clinical and Laboratory Immunology
Volume29
Issue number2
Publication statusPublished - 1989

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Diffuse Scleroderma
Pokeweed Mitogens
Lymphocyte Activation
B-Lymphocytes
Immunoglobulin A
Immunoglobulin M
Immunoglobulin G
Blood Cells
Mitogens
Immunoglobulins
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Famularo, G., Giacomelli, R., Alesse, E., Cifone, M. G., Morrone, S., Boirivant, M., ... Tonietti, G. (1989). Polyclonal B lymphocyte activation in progressive systemic sclerosis. Journal of Clinical and Laboratory Immunology, 29(2), 59-63.

Polyclonal B lymphocyte activation in progressive systemic sclerosis. / Famularo, G.; Giacomelli, R.; Alesse, E.; Cifone, M. G.; Morrone, S.; Boirivant, M.; Danese, C.; Perego, M. A.; Santoni, A.; Tonietti, G.

In: Journal of Clinical and Laboratory Immunology, Vol. 29, No. 2, 1989, p. 59-63.

Research output: Contribution to journalArticle

Famularo, G, Giacomelli, R, Alesse, E, Cifone, MG, Morrone, S, Boirivant, M, Danese, C, Perego, MA, Santoni, A & Tonietti, G 1989, 'Polyclonal B lymphocyte activation in progressive systemic sclerosis', Journal of Clinical and Laboratory Immunology, vol. 29, no. 2, pp. 59-63.
Famularo G, Giacomelli R, Alesse E, Cifone MG, Morrone S, Boirivant M et al. Polyclonal B lymphocyte activation in progressive systemic sclerosis. Journal of Clinical and Laboratory Immunology. 1989;29(2):59-63.
Famularo, G. ; Giacomelli, R. ; Alesse, E. ; Cifone, M. G. ; Morrone, S. ; Boirivant, M. ; Danese, C. ; Perego, M. A. ; Santoni, A. ; Tonietti, G. / Polyclonal B lymphocyte activation in progressive systemic sclerosis. In: Journal of Clinical and Laboratory Immunology. 1989 ; Vol. 29, No. 2. pp. 59-63.
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AU - Boirivant, M.

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