Polyglycolic acid-polylactic acid scaffold response to different progenitor cell in vitro cultures: A demonstrative and comparative X-ray synchrotron radiation phase-contrast microtomography study

Alessandra Giuliani, Francesca Moroncini, Serena Mazzoni, Marzia Laura Chiara Belicchi, Chiara Villa, Silvia Erratico, Elena Colombo, Francesca Calcaterra, Lucia Brambilla, Yvan Torrente, Gianni Albertini, Silvia Della Bella

Research output: Contribution to journalArticle

Abstract

Spatiotemporal interactions play important roles in tissue development and function, especially in stem cell-seeded bioscaffolds. Cells interact with the surface of bioscaffold polymers and influence material-driven control of cell differentiation. In vitro cultures of different human progenitor cells, that is, endothelial colony-forming cells (ECFCs) from a healthy control and a patient with Kaposi sarcoma (an angioproliferative disease) and human CD133+ muscle-derived stem cells (MSH 133+ cells), were seeded onto polyglycolic acid-polylactic acid scaffolds. Three-dimensional (3D) images were obtained by X-ray phase-contrast microtomography (micro-CT) and processed with the Modified Bronnikov Algorithm. The method enabled high spatial resolution detection of the 3D structural organization of cells on the bioscaffold and evaluation of the way and rate at which cells modified the construct at different time points from seeding. The different cell types displayed significant differences in the proliferation rate. In conclusion, X-ray synchrotron radiation phase-contrast micro-CT analysis proved to be a useful and sensitive tool to investigate the spatiotemporal pattern of progenitor cell organization on a bioscaffold.

Original languageEnglish
Pages (from-to)308-316
Number of pages9
JournalTissue Engineering - Part C: Methods
Volume20
Issue number4
DOIs
Publication statusPublished - Apr 1 2014

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Polyglycolic Acid
Synchrotrons
Synchrotron radiation
Stem cells
Scaffolds
Stem Cells
X-Rays
Radiation
Melanocyte-Stimulating Hormones
X rays
Acids
Muscle
Polymers
Tissue
Three-Dimensional Imaging
Kaposi's Sarcoma
poly(lactic acid)
In Vitro Techniques
Cell Differentiation
Muscles

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering
  • Medicine (miscellaneous)

Cite this

Polyglycolic acid-polylactic acid scaffold response to different progenitor cell in vitro cultures : A demonstrative and comparative X-ray synchrotron radiation phase-contrast microtomography study. / Giuliani, Alessandra; Moroncini, Francesca; Mazzoni, Serena; Belicchi, Marzia Laura Chiara; Villa, Chiara; Erratico, Silvia; Colombo, Elena; Calcaterra, Francesca; Brambilla, Lucia; Torrente, Yvan; Albertini, Gianni; Della Bella, Silvia.

In: Tissue Engineering - Part C: Methods, Vol. 20, No. 4, 01.04.2014, p. 308-316.

Research output: Contribution to journalArticle

Giuliani, Alessandra ; Moroncini, Francesca ; Mazzoni, Serena ; Belicchi, Marzia Laura Chiara ; Villa, Chiara ; Erratico, Silvia ; Colombo, Elena ; Calcaterra, Francesca ; Brambilla, Lucia ; Torrente, Yvan ; Albertini, Gianni ; Della Bella, Silvia. / Polyglycolic acid-polylactic acid scaffold response to different progenitor cell in vitro cultures : A demonstrative and comparative X-ray synchrotron radiation phase-contrast microtomography study. In: Tissue Engineering - Part C: Methods. 2014 ; Vol. 20, No. 4. pp. 308-316.
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