TY - JOUR
T1 - Polymer Nanoparticle Engineering for Podocyte Repair
T2 - From in Vitro Models to New Nanotherapeutics in Kidney Diseases
AU - Colombo, Claudio
AU - Li, Min
AU - Watanabe, Shojiro
AU - Messa, Piergiorgio
AU - Edefonti, Alberto
AU - Montini, Giovanni
AU - Moscatelli, Davide
AU - Rastaldi, Maria Pia
AU - Cellesi, Francesco
PY - 2017/2/28
Y1 - 2017/2/28
N2 - Specific therapeutic targeting of kidney podocytes, the highly differentiated ramified glomerular cells involved in the onset and/or progression of proteinuric diseases, could become the optimal strategy for preventing chronic kidney disease. With this aim, we developed a library of engineered polymeric nanoparticles (NPs) of tuneable size and surface properties and evaluated their interaction with podocytes. NP cytotoxicity, uptake, and cytoskeletal effects on podocytes were first assessed. On the basis of these data, nanodelivery of dexamethasone loaded into selected biocompatible NPs was successful in repairing damaged podocytes. Finally, a three-dimensional in vitro system of co-culture of endothelial cells and podocytes was exploited as a new tool for mimicking the mechanisms of NP interaction with glomerular cells and the repair of the kidney filtration barrier.
AB - Specific therapeutic targeting of kidney podocytes, the highly differentiated ramified glomerular cells involved in the onset and/or progression of proteinuric diseases, could become the optimal strategy for preventing chronic kidney disease. With this aim, we developed a library of engineered polymeric nanoparticles (NPs) of tuneable size and surface properties and evaluated their interaction with podocytes. NP cytotoxicity, uptake, and cytoskeletal effects on podocytes were first assessed. On the basis of these data, nanodelivery of dexamethasone loaded into selected biocompatible NPs was successful in repairing damaged podocytes. Finally, a three-dimensional in vitro system of co-culture of endothelial cells and podocytes was exploited as a new tool for mimicking the mechanisms of NP interaction with glomerular cells and the repair of the kidney filtration barrier.
UR - http://www.scopus.com/inward/record.url?scp=85017546210&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85017546210&partnerID=8YFLogxK
U2 - 10.1021/acsomega.6b00423
DO - 10.1021/acsomega.6b00423
M3 - Article
AN - SCOPUS:85017546210
VL - 2
SP - 599
EP - 610
JO - Omega
JF - Omega
SN - 0305-0483
IS - 2
ER -