Polymerase chain reaction, virus isolation and antigen assay in HIV-1-antibody-positive mothers and their children

Gabriella Scarlatti, Valter Lombardi, Anna Plebani, Nicola Principi, Chiara Vegni, Gabriele Ferraris, Anna Bucceri, Eva Maria Fenyö, Hans Wigzell, Paolo Rossi, Jan Albert

Research output: Contribution to journalArticle

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Abstract

Diagnosis of perinatal HIV-1 infection is complicated by the persistence of maternal antibodies and the conflicting reports on polymerase chain reaction (PCR) reactivity in children born to HIV-1-seropositive mothers. We have compared PCR with other diagnostic methods for perinatal HIV-1 infection and have attempted also to identify maternal markers which correlate with risk of transmission. PCR was the most sensitive method for early diagnosis of perinatal transmission of HIV-1, but the PCR-positive children (n = 11) developed at least one additional sign of infection. The PCR-negative children (n = 76) were clinically healthy, virus isolation negative, and their serum was HIV-1-antigen-negative. All children who had become seronegative (n = 36) were both PCR- and isolation-negative. Antigenaemia in the mothers correlated significantly with higher risk of perinatal transmission of HIV-1, while no other parameters (clinical stage, lymphocyte subsets, PCR and isolation) showed such a correlation. This indicates that the level of virus expression may be of key importance for the risk of vertical transmission of HIV-1 infection.

Original languageEnglish
Pages (from-to)1173-1178
Number of pages6
JournalAIDS (London, England)
Volume5
Issue number10
Publication statusPublished - Oct 1991

Fingerprint

HIV Antibodies
HIV-1
Mothers
Viruses
Antigens
Polymerase Chain Reaction
HIV Infections
HIV Antigens
Lymphocyte Subsets
Early Diagnosis
Antibodies
Infection
Serum

Keywords

  • AIDS
  • Antigenaemia
  • HIV-1
  • Mother-to-child
  • Perinatal
  • Polymerase chain reaction
  • Vertical transmission
  • Virus isolation

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Scarlatti, G., Lombardi, V., Plebani, A., Principi, N., Vegni, C., Ferraris, G., ... Albert, J. (1991). Polymerase chain reaction, virus isolation and antigen assay in HIV-1-antibody-positive mothers and their children. AIDS (London, England), 5(10), 1173-1178.

Polymerase chain reaction, virus isolation and antigen assay in HIV-1-antibody-positive mothers and their children. / Scarlatti, Gabriella; Lombardi, Valter; Plebani, Anna; Principi, Nicola; Vegni, Chiara; Ferraris, Gabriele; Bucceri, Anna; Fenyö, Eva Maria; Wigzell, Hans; Rossi, Paolo; Albert, Jan.

In: AIDS (London, England), Vol. 5, No. 10, 10.1991, p. 1173-1178.

Research output: Contribution to journalArticle

Scarlatti, G, Lombardi, V, Plebani, A, Principi, N, Vegni, C, Ferraris, G, Bucceri, A, Fenyö, EM, Wigzell, H, Rossi, P & Albert, J 1991, 'Polymerase chain reaction, virus isolation and antigen assay in HIV-1-antibody-positive mothers and their children', AIDS (London, England), vol. 5, no. 10, pp. 1173-1178.
Scarlatti, Gabriella ; Lombardi, Valter ; Plebani, Anna ; Principi, Nicola ; Vegni, Chiara ; Ferraris, Gabriele ; Bucceri, Anna ; Fenyö, Eva Maria ; Wigzell, Hans ; Rossi, Paolo ; Albert, Jan. / Polymerase chain reaction, virus isolation and antigen assay in HIV-1-antibody-positive mothers and their children. In: AIDS (London, England). 1991 ; Vol. 5, No. 10. pp. 1173-1178.
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