Abstract
A panel of homozygous cell lines, previously typed by primed lymphocyte test for their DPw specificity, have been studied by restriction fragment length polymorphism analysis, using a DPβ-specific probe. Highly stringent hybridization and washing conditions were used to prevent cross-hybridization with DR- and DQ-specific fragments. Three out of six enzymes employed allowed us to distinguish clustered or single DPw specificities, and by MspI digestion it was possible to detect different patterns within a single specificity such as DPw4. Some of the cell lines have been further studied with synthetic oligonucleotides derived from the polymorphic regions of the second exon of DPβ1 gene, and, in general, a correlation with the primed lymphocyte test-defined specificities and restriction fragment length polymorphism was found. These data suggest a more extended complexity of the DP region, in addition to that defined as the DPw1-DPw6 segregant series.
| Original language | English |
|---|---|
| Pages (from-to) | 348-359 |
| Number of pages | 12 |
| Journal | Human Immunology |
| Volume | 27 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1990 |
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ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Polymorphism of the HLA-DPβ region detected by southern blot hybridization. / Chimini, Giovanna; Angelini, Giovanna; Piccioli, Patrizia; Parodi, Barbara; de Totero, Daniela; Battista Ferrara, Giovanni.
In: Human Immunology, Vol. 27, No. 4, 1990, p. 348-359.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Polymorphism of the HLA-DPβ region detected by southern blot hybridization
AU - Chimini, Giovanna
AU - Angelini, Giovanna
AU - Piccioli, Patrizia
AU - Parodi, Barbara
AU - de Totero, Daniela
AU - Battista Ferrara, Giovanni
PY - 1990
Y1 - 1990
N2 - A panel of homozygous cell lines, previously typed by primed lymphocyte test for their DPw specificity, have been studied by restriction fragment length polymorphism analysis, using a DPβ-specific probe. Highly stringent hybridization and washing conditions were used to prevent cross-hybridization with DR- and DQ-specific fragments. Three out of six enzymes employed allowed us to distinguish clustered or single DPw specificities, and by MspI digestion it was possible to detect different patterns within a single specificity such as DPw4. Some of the cell lines have been further studied with synthetic oligonucleotides derived from the polymorphic regions of the second exon of DPβ1 gene, and, in general, a correlation with the primed lymphocyte test-defined specificities and restriction fragment length polymorphism was found. These data suggest a more extended complexity of the DP region, in addition to that defined as the DPw1-DPw6 segregant series.
AB - A panel of homozygous cell lines, previously typed by primed lymphocyte test for their DPw specificity, have been studied by restriction fragment length polymorphism analysis, using a DPβ-specific probe. Highly stringent hybridization and washing conditions were used to prevent cross-hybridization with DR- and DQ-specific fragments. Three out of six enzymes employed allowed us to distinguish clustered or single DPw specificities, and by MspI digestion it was possible to detect different patterns within a single specificity such as DPw4. Some of the cell lines have been further studied with synthetic oligonucleotides derived from the polymorphic regions of the second exon of DPβ1 gene, and, in general, a correlation with the primed lymphocyte test-defined specificities and restriction fragment length polymorphism was found. These data suggest a more extended complexity of the DP region, in addition to that defined as the DPw1-DPw6 segregant series.
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U2 - 10.1016/0198-8859(90)90085-4
DO - 10.1016/0198-8859(90)90085-4
M3 - Article
VL - 27
SP - 348
EP - 359
JO - Human Immunology
JF - Human Immunology
SN - 0198-8859
IS - 4
ER -