TY - JOUR
T1 - Polymorphisms in DNA repair genes
T2 - Link with biomarkers of the CBMN cytome assay in hospital workers chronically exposed to low doses of ionising radiation
AU - Milić, Mirta
AU - Rozgaj, Ružica
AU - Kašuba, Vilena
AU - Jazbec, Ana Marija
AU - Starčević, Boris
AU - Lyzbicki, Barnaba
AU - Ravegnini, Gloria
AU - Zenesini, Corrado
AU - Musti, Muriel
AU - Hrelia, Patrizia
AU - Angelini, Sabrina
PY - 2015/6/1
Y1 - 2015/6/1
N2 - Individual sensitivity to ionising radiation (IR) is the result of interaction between exposure, DNA damage, and its repair, which is why polymorphisms in DNA repair genes could play an important role. We examined the association between DNA damage, expressed as micronuclei (MNi), nuclear buds (NBs), and nucleoplasmic bridges (NPBs) and single nucleotide polymorphisms in selected DNA repair genes (APE1, hOGG1, XRCC1, XRCC3, XPD, PARP1, MGMT genes; representative of the different DNA repair pathways operating in mammals) in 77 hospital workers chronically exposed to low doses of IR, and 70 matched controls. A significantly higher MNi frequency was found in the exposed group (16.2±10.4 vs. 11.5±9.4; P=0.003) and the effect appeared to be independent from the principal confounding factor. Exposed individuals with hOGG1, XRCC1, PARP1, and MGMT wild-type alleles or APEX1, as well as XPD (rs13181) heterozygous showed a significantly higher MNi frequency than controls with the same genotypes. Genetic polymorphism analysis and cytogenetic dosimetry have proven to be a powerful tool complementary to physical dosimetry in regular health surveillance programmes.
AB - Individual sensitivity to ionising radiation (IR) is the result of interaction between exposure, DNA damage, and its repair, which is why polymorphisms in DNA repair genes could play an important role. We examined the association between DNA damage, expressed as micronuclei (MNi), nuclear buds (NBs), and nucleoplasmic bridges (NPBs) and single nucleotide polymorphisms in selected DNA repair genes (APE1, hOGG1, XRCC1, XRCC3, XPD, PARP1, MGMT genes; representative of the different DNA repair pathways operating in mammals) in 77 hospital workers chronically exposed to low doses of IR, and 70 matched controls. A significantly higher MNi frequency was found in the exposed group (16.2±10.4 vs. 11.5±9.4; P=0.003) and the effect appeared to be independent from the principal confounding factor. Exposed individuals with hOGG1, XRCC1, PARP1, and MGMT wild-type alleles or APEX1, as well as XPD (rs13181) heterozygous showed a significantly higher MNi frequency than controls with the same genotypes. Genetic polymorphism analysis and cytogenetic dosimetry have proven to be a powerful tool complementary to physical dosimetry in regular health surveillance programmes.
KW - genotype analysis
KW - micronucleus
KW - nuclear bud
KW - nucleoplasmic bridge
KW - occupational exposure
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U2 - 10.1515/aiht-2015-66-2655
DO - 10.1515/aiht-2015-66-2655
M3 - Article
AN - SCOPUS:84934759112
VL - 66
SP - 109
EP - 120
JO - Arhiv za Higijenu Rada i Toksikologiju
JF - Arhiv za Higijenu Rada i Toksikologiju
SN - 0004-1254
IS - 2
ER -