Polymorphisms in the promoter region and at codon 54 of the MBL2 gene are not associated with IgA nephropathy

Doroti Pirulli, Michele Boniotto, Laura Vatta, Sergio Crovella, Andrea Spano, Marcello Morgutti, Silvia Zezlina, Laura Bertola, Dario Roccatello, Francesco Scolari, Licia Peruzzi, Silvana Savoldi, Antonio Amoroso

Research output: Contribution to journalArticle

Abstract

Background. IgA nephropathy (IgAN) occurs sporadically in unrelated individuals. Several different polymorphic genes have been investigated in recent years in order to demonstrate their possible association with IgAN. Three recent, different studies with conflicting conclusions have discussed the role of the mannose binding lectin (MBL), a serum lectin involved in natural immunity, in the IgAN pathogenesis by examination of MBL deposits in biopsies. In the present study we investigated several polymorphisms of the MBL gene located in the promoter region and in the first exon. Methods. MBL polymorphism detection was performed in 22 Italian patients with familial IgA nephropathy and in 138 Italian patients with the sporadic form of the disease. The polymorphisms in the MBL2 promoter region and in the exon 1 were investigated, respectively, by direct sequencing and by amplification refractory mutation system-polymerase chain reaction on genomic DNA collected from peripheral blood. Seventy-four unrelated healthy subjects matched for ethnic origin were used as controls. Results. Allelic and genotypic frequencies of the polymorphisms at position -550, -328, -221 and at codon 54 did not show any differences between patients and controls. Similar frequency distributions of these polymorphisms were also found in the subgroups of IgAN patients subdivided according to the clinical manifestations and the progression of the disease. Conclusions. This study indicates that the analysed polymorphisms of the MBL gene do not appear to be primarily involved in the susceptibility and severity of IgAN.

Original languageEnglish
Pages (from-to)759-764
Number of pages6
JournalNephrology Dialysis Transplantation
Volume16
Issue number4
Publication statusPublished - 2001

Keywords

  • DNA
  • Genetics
  • IGA nephoropathy
  • MBL2
  • Polymorphisms
  • Susceptibility

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

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    Pirulli, D., Boniotto, M., Vatta, L., Crovella, S., Spano, A., Morgutti, M., Zezlina, S., Bertola, L., Roccatello, D., Scolari, F., Peruzzi, L., Savoldi, S., & Amoroso, A. (2001). Polymorphisms in the promoter region and at codon 54 of the MBL2 gene are not associated with IgA nephropathy. Nephrology Dialysis Transplantation, 16(4), 759-764.