Polyomavirus JC-targeted T-cell therapy for progressive multiple leukoencephalopathy in a hematopoietic cell transplantation recipient

A. Balduzzi, G. Lucchini, H. H. Hirsch, S. Basso, M. Cioni, A. Rovelli, A. Zincone, M. Grimaldi, P. Corti, S. Bonanomi, A. Biondi, F. Locatelli, E. Biagi, P. Comoli

Research output: Contribution to journalArticle

Abstract

Progressive multifocal leukoencephalopathy (PML) associated with polyomavirus JC (JCV) infection has been reported to be usually fatal in allogeneic hematopoietic SCT (HSCT) recipients. We present the case of a 19-year-old HSCT patient diagnosed with JCV-associated PML after prolonged immunosuppression for severe GVHD. No short-term neurological improvement was observed after antiviral treatment and discontinuation of immunosuppressive therapy. Donor-derived JCV Ag-specific CTLs were generated in vitro after stimulation with 15-mer peptides derived from VP1 and large T viral proteins. After adoptive CTL infusion, virus-specific cytotoxic cells were shown in the peripheral blood, JCV-DNA was cleared in the cerebrospinal fluid and the patient showed remarkable improvement. Adoptive T-lymphocyte therapy with JCV-specific CTLs was feasible and had no side effects. This case suggests that adoptive transfer of JCV-targeted CTLs may contribute to restore JCV-specific immune competence and control PML in transplanted patients.

Original languageEnglish
Pages (from-to)987-992
Number of pages6
JournalBone Marrow Transplantation
Volume46
Issue number7
DOIs
Publication statusPublished - Jul 2011

Keywords

  • adoptive Immunotherapy
  • allogeneic transplantation
  • CTL
  • GVHD
  • polyomavirus JC
  • progressive multifocal leukoencephalopathy

ASJC Scopus subject areas

  • Hematology
  • Transplantation

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  • Cite this

    Balduzzi, A., Lucchini, G., Hirsch, H. H., Basso, S., Cioni, M., Rovelli, A., Zincone, A., Grimaldi, M., Corti, P., Bonanomi, S., Biondi, A., Locatelli, F., Biagi, E., & Comoli, P. (2011). Polyomavirus JC-targeted T-cell therapy for progressive multiple leukoencephalopathy in a hematopoietic cell transplantation recipient. Bone Marrow Transplantation, 46(7), 987-992. https://doi.org/10.1038/bmt.2010.221