Polypeptide specificity of antiviral serum antibodies in children naturally infected with human rotavirus

H. Brussow; P. A. Offit; G. Gerna; A. Bruttin; J. Sidoti

Polypeptide specificity of antiviral serum antibodies in children naturally infected with human rotavirus

H. Brussow, P. A. Offit, G. Gerna, A. Bruttin, J. Sidoti

IRCCS Fondazione Policlinico San Matteo

Research output: Contribution to journal › Article

Abstract

Reassortants between serotype 3 SA11 and serotype 6 NCDV rotaviruses were used to determine the relative amounts of serum-neutralizing antibody to VP4 and VP7 of serotype 3 SA11 rotavirus in children after natural rotavirus exposure. Sera from Ecuadorian children of a population-based study and sera from children of a hospital-based study in Germany (excluding diarrhea patients) demonstrated high titers of VP7-specific but only low titers of VP4-specific antibodies. In contrast, paired sera from German children hospitalized with a symptomatic primary rotavirus gastroenteritis demonstrated a titer increase to VP4 more frequently than to VP7 protein by neutralization test and immunoblotting. For these rotavirus patients, we provided, previously, direct evidence for the development of cross-neutralizing antibodies.

Original language	English
Pages (from-to)	4130-4136
Number of pages	7
Journal	Journal of Virology
Volume	64
Issue number	9
Publication status	Published - 1990

ASJC Scopus subject areas

Immunology

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Brussow, H., Offit, P. A., Gerna, G., Bruttin, A., & Sidoti, J. (1990). Polypeptide specificity of antiviral serum antibodies in children naturally infected with human rotavirus. Journal of Virology, 64(9), 4130-4136.

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abstract = "Reassortants between serotype 3 SA11 and serotype 6 NCDV rotaviruses were used to determine the relative amounts of serum-neutralizing antibody to VP4 and VP7 of serotype 3 SA11 rotavirus in children after natural rotavirus exposure. Sera from Ecuadorian children of a population-based study and sera from children of a hospital-based study in Germany (excluding diarrhea patients) demonstrated high titers of VP7-specific but only low titers of VP4-specific antibodies. In contrast, paired sera from German children hospitalized with a symptomatic primary rotavirus gastroenteritis demonstrated a titer increase to VP4 more frequently than to VP7 protein by neutralization test and immunoblotting. For these rotavirus patients, we provided, previously, direct evidence for the development of cross-neutralizing antibodies.",

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AU - Brussow, H.

AU - Offit, P. A.

AU - Gerna, G.

AU - Bruttin, A.

AU - Sidoti, J.

PY - 1990

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AB - Reassortants between serotype 3 SA11 and serotype 6 NCDV rotaviruses were used to determine the relative amounts of serum-neutralizing antibody to VP4 and VP7 of serotype 3 SA11 rotavirus in children after natural rotavirus exposure. Sera from Ecuadorian children of a population-based study and sera from children of a hospital-based study in Germany (excluding diarrhea patients) demonstrated high titers of VP7-specific but only low titers of VP4-specific antibodies. In contrast, paired sera from German children hospitalized with a symptomatic primary rotavirus gastroenteritis demonstrated a titer increase to VP4 more frequently than to VP7 protein by neutralization test and immunoblotting. For these rotavirus patients, we provided, previously, direct evidence for the development of cross-neutralizing antibodies.

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