Polyphenols-Loaded Sericin Self-Assembling Nanoparticles: A Slow-Release for Regeneration by Tissue-Resident Mesenchymal Stem/Stromal Cells

Giulia Orlandi, Elia Bari, Laura Catenacci, Milena Sorrenti, Lorena Segale, Silvio Faragò, Marzio Sorlini, Carla Renata Arciola, Maria Luisa Torre, Sara Perteghella

Research output: Contribution to journalArticlepeer-review

Abstract

Mesenchymal stem/stromal cells (MSCs) are a therapeutic target to promote tissue regeneration, mainly when oxidative stress-mediated damage is involved in disease pathogenesis. Here, slow-release silk sericin nanoparticles (SNPs) loaded with natural antioxidant polyphenols were developed to sustain regeneration by tissue-resident MSCs. SNPs were prepared by exploiting a self-assembly method with poloxamer and were loaded with proanthocyanidins (P), quercetin (Q) or epigallocatechin gallate (E). SNPs, with a diameter less than 150 nm, were able to encapsulate both hydrophilic (P and E) and hydrophobic (Q) drugs. A slow and controlled release was obtained from SNPs for all the actives in PBS, while in EtOH, Q and E showed a burst release but P did not. Kinetic models revealed lower diffusion of P than other biomolecules, probably due to the higher steric hindrance of P. The in vitro anti-oxidant, anti-elastase and anti-tyrosinase properties of SNPs were assessed: loading the P and E into SNPs preserved the in vitro biological activities whereas for Q, the anti-elastase activity was strongly improved. Moreover, all formulations promoted MSC metabolic activity over 72 h. Finally, SNPs exhibited a strong ability to protect MSCs from oxidative stress, which supports their potential use for regenerative purposes mediated by tissue-resident MSCs.

Original languageEnglish
Pages (from-to)1-24
Number of pages24
JournalPharmaceutics
Volume12
Issue number4
DOIs
Publication statusPublished - Apr 21 2020

Keywords

  • silk-sericin nanoparticles
  • proanthocyanidins
  • quercetin
  • epigallocatechin gallate
  • tissue regeneration
  • mesenchymal stem/stromal cells

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