Pooled human serum: A new culture supplement for bioreactor-based cell therapies. Preliminary results

Sara Savelli, Luisa Trombi, Delfo D'Alessandro, Stefania Moscato, Simone Pacini, Stefano Giannotti, Simone Lapi, Fabrizio Scatena, Mario Petrini

Research output: Contribution to journalArticle

Abstract

Background: Bone Marrow MSCs are an appealing source for several cell-based therapies. Many bioreactors, as the Quantum Cell Expansion System, have been developed to generate a large number of MSCs under Good Manufacturing Practice conditions by using Human Platelet Lysate (HPL). Previously we isolated in the human bone marrow a novel cell population, named Mesodermal Progenitor Cells (MPCs), which we identified as precursors of MSCs. MPCs could represent an important cell source for regenerative medicine applications. As HPL gives rise to a homogeneus MSC population, limiting the harvesting of other cell types, in this study we investigated the efficacy of pooled human AB serum (ABS) to provide clinically relevant numbers of both MSCs and MPCs for regenerative medicine applications by using the Quantum System. Methods: Bone marrow aspirates were obtained from healthy adult individuals undergoing routine total hip replacement surgery and used to generate primary cultures in the bioreactor. HPL and ABS were tested as supplements to culture medium. Morphological observations, cytofluorimetric analysis, lactate and glucose level assessment were performed. Results: ABS gave rise to both heterogeneous MSC and MPC population. About 95% of cells cultured in HPL showed a fibroblast-like morphology and typical mesenchymal surface markers, but MPCs were scarcely represented. Discussion: The use of ABS appeared to sustain a large scale MSC production, as well as the recovery of a subset of MPCs, and resulted a suitable alternative to HPL in the cell generation based on the Quantum System.

Original languageEnglish
Pages (from-to)556-563
Number of pages8
JournalCytotherapy
Volume20
Issue number4
DOIs
Publication statusPublished - Apr 1 2018

Keywords

  • bioreactors
  • bone marrow derived MSCs
  • large-scale cell manufacturing
  • xeno-free supplements

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Genetics(clinical)
  • Cell Biology
  • Transplantation
  • Cancer Research

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    Savelli, S., Trombi, L., D'Alessandro, D., Moscato, S., Pacini, S., Giannotti, S., Lapi, S., Scatena, F., & Petrini, M. (2018). Pooled human serum: A new culture supplement for bioreactor-based cell therapies. Preliminary results. Cytotherapy, 20(4), 556-563. https://doi.org/10.1016/j.jcyt.2017.12.013