Porous composite scaffolds based on gelatin and partially hydrolyzed α-tricalcium phosphate

S. Panzavolta, M. Fini, A. Nicoletti, B. Bracci, K. Rubini, R. Giardino, A. Bigi

Research output: Contribution to journalArticle

Abstract

Porous composite scaffolds of varying compositions were prepared by freeze-drying gelatin foams containing increasing amounts of α-tricalcium phosphate (α-TCP), up to about 40 wt.%. Due to the presence of gelatin, a partial hydrolysis of α-TCP into octacalcium phosphate (OCP) occurs during foaming. As a consequence, the scaffolds contain both α-TCP and OCP, in relative amounts of about 74% and 26%, respectively, independent of the initial composition. In physiological conditions the inorganic component of the scaffolds undergoes a further hydrolysis as shown by the finding that after immersion in phosphate-buffered saline at 37 °C for 1 week the scaffolds contain poorly crystalline hydroxyapatite together with OCP. The scaffolds display a porous interconnected microstructure. The mean dimensions of the pores decrease from about 350 to about 170 μm as the inorganic phase content increases. Simultaneously, the mean values of the compression strength and Young's modulus increase. Stabilization of the scaffolds was obtained by using a natural, non-toxic, crosslinking agent, genipin, which significantly improves their mechanical properties.

Original languageEnglish
Pages (from-to)636-643
Number of pages8
JournalActa Biomaterialia
Volume5
Issue number2
DOIs
Publication statusPublished - Feb 2009

Fingerprint

Gelatin
Scaffolds
Phosphates
Composite materials
Hydrolysis
Freeze Drying
Elastic Modulus
Immersion
Durapatite
Chemical analysis
Hydroxyapatite
Crosslinking
Foams
tricalcium phosphate
octacalcium phosphate
Drying
Stabilization
Elastic moduli
Crystalline materials
Mechanical properties

Keywords

  • α-Tricalcium phosphate
  • Bone scaffold
  • Gelatin
  • Octacalcium phosphate

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Molecular Biology
  • Biomedical Engineering
  • Biomaterials

Cite this

Porous composite scaffolds based on gelatin and partially hydrolyzed α-tricalcium phosphate. / Panzavolta, S.; Fini, M.; Nicoletti, A.; Bracci, B.; Rubini, K.; Giardino, R.; Bigi, A.

In: Acta Biomaterialia, Vol. 5, No. 2, 02.2009, p. 636-643.

Research output: Contribution to journalArticle

Panzavolta, S, Fini, M, Nicoletti, A, Bracci, B, Rubini, K, Giardino, R & Bigi, A 2009, 'Porous composite scaffolds based on gelatin and partially hydrolyzed α-tricalcium phosphate', Acta Biomaterialia, vol. 5, no. 2, pp. 636-643. https://doi.org/10.1016/j.actbio.2008.08.017
Panzavolta, S. ; Fini, M. ; Nicoletti, A. ; Bracci, B. ; Rubini, K. ; Giardino, R. ; Bigi, A. / Porous composite scaffolds based on gelatin and partially hydrolyzed α-tricalcium phosphate. In: Acta Biomaterialia. 2009 ; Vol. 5, No. 2. pp. 636-643.
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