Porous silicon films micropatterned with bioelements as supports for mammalian cells

Martin J. Sweetman, Maurizio Ronci, Soraya Rasi Ghaemi, Jamie E. Craig, Nicolas H. Voelcker

Research output: Contribution to journalArticle

Abstract

Porous silicon (pSi) surfaces have been chemically patterned via a UV initiated hydrosilylation reaction of an alkene through a photomask, introducing chemical functionality in the exposed surface areas. A secondary, UV initiated hydrosilylation reaction with a second alkene of different functionality is performed to backfill the silicon hydride terminated regions on the surface, thereby affording patterned porous films with dual, surface chemistry. UV initiated hydrosilylations were performed using the alkene undecylenic acid N-hydroxysuccinimide (NHS) ester, and the pSi surfaces were stabilized by a second hydrosilylation reaction with a polyethylene glycol (PEG) appended alkene. NHS ester and PEG functionalized surfaces were used for the selective immobilization of the cell adhesion mediator protein fibronectin (FN), in the NHS-functional regions. Matrix-assisted laser desorption/ionization mass spectrometry imaging on the protein functionalized pSi surface confirmed the patterned conjugation of the FN to the NHS functionalized regions. Mammalian cells cultured on these surfaces showed attachment that was confined to the patterned areas of FN on the pSi surface.

Original languageEnglish
Pages (from-to)1158-1166
Number of pages9
JournalAdvanced Functional Materials
Volume22
Issue number6
DOIs
Publication statusPublished - Mar 21 2012

Keywords

  • biomaterials
  • cell culture
  • hydrosilylation
  • patterning
  • porous silicon

ASJC Scopus subject areas

  • Biomaterials
  • Electrochemistry
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

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