Positive identification in situ of mRNA expression of IL-6, and IL-12, and the chemotactic cytokine RANTES in patients with chronic sinusitis and polypoid disease. Clinical relevance and relation to allergy

Åke ̊ Davidsson, Arild Danielsen, Giuseppe Viale, Jan Olofsson, Patrizia Dell'orto, Caterina Pellegrini, Mats G. Karlsson, Henrik B. Hellquist

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Interleukins 6 (IL-6) and 12 (IL-12), and the chemoattractant chemokine RANTES were studied in ethmoidal mucosa, using reverse transcriptase polymerase chain reaction. The 49 patients had chronic sinusitis or nasal/paranasal polyposis, and some also allergy. To the best of our knowledge, this is the first study that demonstrates RANTES and IL-12 on mRNA level in human sinonasal mucosa in situ. mRNA for IL-6, IL-12 and RANTES were detected in 2, 8 and 6 patients with chronic sinusitis, respectively, and in mucosa from patients with polyposis a positive expression was observed in 4, 14 and 10 cases. There were no statistically significant differences. Analysing the entire group of 49 patients, disregarding type of mucosal disease, the number of patients with positive RANTES was significantly higher than that for IL-6. Similarly, IL-12 positivity was more frequently expressed than IL-6, mRNA for IL-6 was expressed in only 2 of the allergic patients. The cytokine production studied thus seems to be unrelated to the clinically defined entities. There is thus a local production in human diseased sinonasal mucosa of RANTES, as well as of IL-6 and IL-12. The local production of RANTES is an important prerequisite for recruitment and migration of inflammatory cells into the tissue. IL-12 is a co-stimulator of antigen-specific responses of established T helper 1 (Th1) clones, and regulates the responsiveness of the clones to a number of T cell growth factors. The study supports a shift towards Th1 cells in these disease entities.

Original languageEnglish
Pages (from-to)604-610
Number of pages7
JournalActa Oto-Laryngologica
Volume116
Issue number4
Publication statusPublished - 1996

Fingerprint

Chemokine CCL5
Sinusitis
Interleukin-12
Chemokines
Interleukin-6
Hypersensitivity
Messenger RNA
Mucous Membrane
Clone Cells
Th1 Cells
Chemotactic Factors
Reverse Transcriptase Polymerase Chain Reaction
Nose
Cell Movement
Interleukin-2

Keywords

  • Chemokines
  • Cytokines
  • Inflammatory mediators
  • Nasal polyposis
  • Sinusitis

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Positive identification in situ of mRNA expression of IL-6, and IL-12, and the chemotactic cytokine RANTES in patients with chronic sinusitis and polypoid disease. Clinical relevance and relation to allergy. / Davidsson, Åke ̊; Danielsen, Arild; Viale, Giuseppe; Olofsson, Jan; Dell'orto, Patrizia; Pellegrini, Caterina; Karlsson, Mats G.; Hellquist, Henrik B.

In: Acta Oto-Laryngologica, Vol. 116, No. 4, 1996, p. 604-610.

Research output: Contribution to journalArticle

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abstract = "Interleukins 6 (IL-6) and 12 (IL-12), and the chemoattractant chemokine RANTES were studied in ethmoidal mucosa, using reverse transcriptase polymerase chain reaction. The 49 patients had chronic sinusitis or nasal/paranasal polyposis, and some also allergy. To the best of our knowledge, this is the first study that demonstrates RANTES and IL-12 on mRNA level in human sinonasal mucosa in situ. mRNA for IL-6, IL-12 and RANTES were detected in 2, 8 and 6 patients with chronic sinusitis, respectively, and in mucosa from patients with polyposis a positive expression was observed in 4, 14 and 10 cases. There were no statistically significant differences. Analysing the entire group of 49 patients, disregarding type of mucosal disease, the number of patients with positive RANTES was significantly higher than that for IL-6. Similarly, IL-12 positivity was more frequently expressed than IL-6, mRNA for IL-6 was expressed in only 2 of the allergic patients. The cytokine production studied thus seems to be unrelated to the clinically defined entities. There is thus a local production in human diseased sinonasal mucosa of RANTES, as well as of IL-6 and IL-12. The local production of RANTES is an important prerequisite for recruitment and migration of inflammatory cells into the tissue. IL-12 is a co-stimulator of antigen-specific responses of established T helper 1 (Th1) clones, and regulates the responsiveness of the clones to a number of T cell growth factors. The study supports a shift towards Th1 cells in these disease entities.",
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AU - Davidsson, Åke ̊

AU - Danielsen, Arild

AU - Viale, Giuseppe

AU - Olofsson, Jan

AU - Dell'orto, Patrizia

AU - Pellegrini, Caterina

AU - Karlsson, Mats G.

AU - Hellquist, Henrik B.

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