Positive selection and expansion of cytomegalovirus-specific CD4 and CD8 T cells in sealed systems: Potential applications for adoptive cellular immunoreconstitution

Giuseppina Li Pira, Federico Ivaldi, Gino Tripodi, Marina Martinengo, Fabrizio Manca

Research output: Contribution to journalArticle

Abstract

Administration of pathogen-specific T-cell lines can reconstitute the cellular immune function of immunocompromised patients. Selection and expansion of specific T cells for reinfusion pose unique challenges owing to the fact that good manufacturing procedures must be implemented. Cytokine secretion-based methods can identify and select specific T cells. We showed here that it is possible to combine this method with procedures for cell handling performed in a sealed, unbreached system from start to end. Peripheral blood mononuclear cells, obtained from blood samples and processed in a sealed system, were stimulated in Teflon bags with a library of selected CD4 and CD8 peptides derived from the immunodominant cytomegalovirus protein pp65. The stimulated T cells were labeled with reagents for interferon-γ surface capture and selected on a magnetic column using a sealed system connected to the Teflon bags. Elution and final expansion were also performed with an unbreached protocol with preservation of sterility even if the steps were run on the bench top. Expanded cells exhibited the appropriate functions. The use of this unbreached procedure proves that safety of cellular products generated in a good manufacturing procedures facility can be further improved. Similar sealed protocols can also be applied for T-cell therapies directed against tumor antigens.

Original languageEnglish
Pages (from-to)762-770
Number of pages9
JournalJournal of Immunotherapy
Volume31
Issue number8
DOIs
Publication statusPublished - Oct 2008

Keywords

  • Adoptive immunoreconstitution
  • Cytomegalovirus
  • Sealed culture systems
  • T-cell lines

ASJC Scopus subject areas

  • Cancer Research
  • Immunology
  • Medicine(all)
  • Immunology and Allergy
  • Pharmacology

Fingerprint Dive into the research topics of 'Positive selection and expansion of cytomegalovirus-specific CD4 and CD8 T cells in sealed systems: Potential applications for adoptive cellular immunoreconstitution'. Together they form a unique fingerprint.

  • Cite this