Possible involvement of Ca++ ions, protein kinase C and Na+-H+ antiporter in insulin-induced endogenous dopamine release from tuberoinfundibular neurons

Salvatore Amoroso, Maurizio Taglialatela, Lorella M T Canzoniero, Edward J. Cragoe, Gianfranco di Renzo, Lucio Annunziato

Research output: Contribution to journalArticle

Abstract

Insulin (63 μM) stimulated endogenous dopamine (DA) release from tuberoinfundibular neurons. This effect was independent on the presence of extracellular glucose and did not involve the outward transport of DA, mediated by its membrane carrier. By contrast this effect was completely prevented by the removal of extracellular Ca++ ions in presence of the Ca++- chelator ethyleneglycol-2- (2-aminoethyl)-tetracetic acid (EGTA). Furthermore 1-(5-isoquinolinyl-sulfonyl)-2-methyl-piperazine (H7), a compound which behaves as a putative inhibitor of protein kinase C (PK-C) (10μM), completely counteracted the stimulation of endogenous DA release induced by insulin. Amiloride (300 μM) and its 5-amino nitrogen atom-substituted derivative, 5-(N-methyl-N-(guanidinocarbonylmethyl) amiloride (MGCMA) (10 μM), a highly selective inhibitor of the Na+-H+ membrane antiporter, were both able to prevent the stimulatory action exerted by insulin on endogenous DA release. Collectively, these results suggest that the transductional events by which insulin stimulated endogenous DA release from TIDA neurons may involve the activation of PK-C, the enhancement of Ca++ influx and the stimulation of the Na+-H+ exchange system.

Original languageEnglish
Pages (from-to)885-894
Number of pages10
JournalLife Sciences
Volume46
Issue number12
DOIs
Publication statusPublished - 1990

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Sodium-Hydrogen Antiporter
Protein Kinase C
Neurons
Dopamine
Ions
Insulin
Amiloride
Antiporters
Membranes
Egtazic Acid
Chelating Agents
Nitrogen
Chemical activation
Derivatives
Glucose
Atoms
Acids

ASJC Scopus subject areas

  • Pharmacology

Cite this

Possible involvement of Ca++ ions, protein kinase C and Na+-H+ antiporter in insulin-induced endogenous dopamine release from tuberoinfundibular neurons. / Amoroso, Salvatore; Taglialatela, Maurizio; Canzoniero, Lorella M T; Cragoe, Edward J.; di Renzo, Gianfranco; Annunziato, Lucio.

In: Life Sciences, Vol. 46, No. 12, 1990, p. 885-894.

Research output: Contribution to journalArticle

Amoroso, Salvatore ; Taglialatela, Maurizio ; Canzoniero, Lorella M T ; Cragoe, Edward J. ; di Renzo, Gianfranco ; Annunziato, Lucio. / Possible involvement of Ca++ ions, protein kinase C and Na+-H+ antiporter in insulin-induced endogenous dopamine release from tuberoinfundibular neurons. In: Life Sciences. 1990 ; Vol. 46, No. 12. pp. 885-894.
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