Possible Involvement of the CACNA1E Gene in Migraine: A Search for Single Nucleotide Polymorphism in Different Clinical Phenotypes

Anna Ambrosini, Mara D'Onofrio, Maria Gabriella Buzzi, Ivan Arisi, Gaetano S Grieco, Francesco Pierelli, Filippo M Santorelli, Jean Schoenen

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVE: To search for differences in prevalence of a CACNA1E variant between migraine without aura, various phenotypes of migraine with aura, and healthy controls.

BACKGROUND: Familial hemiplegic migraine type 1 (FHM1) is associated with mutations in the CACNA1A gene coding for the alpha 1A (Cav 2.1) pore-forming subunit of P/Q voltage-dependent Ca2+ channels. These mutations are not found in the common forms of migraine with or without aura. The alpha 1E subunit (Cav 2.3) is the counterpart of Cav 2.1 in R-type Ca2+ channels, has different functional properties, and is encoded by the CACNA1E gene.

METHODS: First, we performed a total exon sequencing of the CACNA1E gene in three probands selected because they had no abnormalities in the three FHM genes. In a patient suffering from basilar-type migraine, we identified a single nucleotide polymorphism (SNP) in exon 20 of the CACNA1E gene (Asp859Glu - rs35737760; Minor Allele Frequency 0.2241) hitherto not studied in migraine. In a second step, we determined its occurrence in four groups by direct sequencing on blood genomic DNA: migraine patients without aura (N = 24), with typical aura (N = 55), complex neurological auras (N = 19; hemiplegic aura: N = 15; brain stem aura: N = 4), and healthy controls (N = 102).

RESULTS: The Asp859Glu - rs35737760 SNP of the CACNA1E gene was present in 12.7% of control subjects and in 20.4% of the total migraine group. In the migraine group it was significantly over-represented in patients with complex neurological auras (42.1%), OR 4.98 (95% CI: 1.69-14.67, uncorrected P = .005, Bonferroni P = .030, 2-tailed Fisher's exact test). There was no significant difference between migraine with typical aura (10.9%) and controls.

CONCLUSIONS: We identified a polymorphism in exon 20 of the CACNA1E gene (Asp859Glu - rs35737760) that is more prevalent in hemiplegic and brain stem aura migraine. This missense variant causes a change from aspartate to glutamate at position 859 of the Cav 2.3 protein and might modulate the function of R-type Ca2+ channels. It could thus be relevant for migraine with complex neurological aura, although this remains to be proven.

Original languageEnglish
Pages (from-to)1136-1144
Number of pages9
JournalHeadache
Volume57
Issue number7
DOIs
Publication statusPublished - Jul 2017

Keywords

  • Aspartic Acid
  • Calcium Channels, R-Type
  • Case-Control Studies
  • Cation Transport Proteins
  • Cerebellar Ataxia
  • DNA Mutational Analysis
  • Exons
  • Female
  • Gene Frequency
  • Genetic Predisposition to Disease
  • Glutamic Acid
  • Humans
  • Male
  • Migraine Disorders
  • Phenotype
  • Polymorphism, Single Nucleotide
  • Retrospective Studies
  • Statistics, Nonparametric
  • Journal Article

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