Possible involvement of tumour cell membrane gangliosides in platelet-tumour cell interactions

P. Ferroni, L. Lenti, F. Guadagni, F. Martini, F. D'Agostino, A. Spila, G. M. Pontieri, P. P. Gazzaniga

Research output: Contribution to journalArticlepeer-review

Abstract

The possible correlation(s) between platelet proaggregating activity, and sialic acid content and ganglioside expression of six human colorectal tumour cell lines (CBS, GEO, HT-29, WiDr, MIP and DLD-1) was evaluated. The three cell lines (HT-29, WiDr and DLD-1) capable of inducing remarkable in vitro platelet aggregation, had significantly higher amounts of lipid-bound sialic acid than those cell lines characterised by a lower platelet proaggregating activity (GEO, CBS and MIP). High performance thin-layer chromatography demonstrated the presence of one band comigrating with GM3 in all cell lines, while GD1a and GT1b comigrating gangliosides were present only in HT-29, WiDr and DLD-1 cells. Finally, an increased platelet pro-aggregating activity of GEO and CBS cell lines was observed after the incorporation of exogenous gangliosides. The present data support the hypothesis that lipid-bound sialic acid may be involved in platelet-tumour cell interactions.

Original languageEnglish
Pages (from-to)79-84
Number of pages6
JournalEuropean Journal of Cancer
Volume31
Issue number1
DOIs
Publication statusPublished - 1995

Keywords

  • colon cancer
  • gangliosides
  • metastases
  • platelet aggregation
  • platelets
  • sialic acid

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Hematology

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