Possible prognostic role of IL-17R in osteosarcoma

Maria Cristina Honorati, Luca Cattini, Andrea Facchini

Research output: Contribution to journalArticle

Abstract

Purpose: An intense vascularization of primary tumor mass is associated with a fatal outcome in various types of invasive solid tumors. Interleukin 17 (IL-17), a CD4+ T-cell-derived cytokine, stimulates some tumor cells to secrete angiogenic factors, among which venous endothelial growth factor (VEGF). We assessed whether the expression of IL-17 receptor (IL-17R) represents a marker for the metastasizing ability of osteosarcoma (OS), a very malignant bone tumor. Methods: We immunoassayed the amount of VEGF secreted by three OS cell lines expressing IL-17R in differing amounts: HOS, MG63 and U-2 OS, and their sensitivity to IL-17 stimulation to secrete VEGF. Results: U-2 OS, which best expresses IL-17R, secreted the highest amounts of VEGF and was the most sensitive to IL-17, whereas MG63 expressed the lowest level of IL-17R, secreted the lowest amount of VEGF and was not sensitive to IL-17. IL-17R expression correlated with VEGF secretion and IL-17 sensitivity. U-2 OS expressed the most dedifferentiated phenotype, which is associated with tumor malignancy. Conclusions: These results suggest that IL-17R in OS might represent a marker of tumor metastasis potential.

Original languageEnglish
Pages (from-to)1017-1021
Number of pages5
JournalJournal of Cancer Research and Clinical Oncology
Volume133
Issue number12
DOIs
Publication statusPublished - Dec 2007

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Keywords

  • IL-17R
  • Malignancy marker
  • Osteosarcoma
  • VEGF

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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