Post-ischaemic long-term synaptic potentiation in the striatum

A putative mechanism for cell type-specific vulnerability

Paolo Calabresi, Emilia Saulle, Diego Centonze, Antonio Pisani, Girolama A. Marfia, Giorgio Bernardi

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

In the present in vitro study of rat brain, we report that transient oxygen and glucose deprivation (in vitro ischaemia) induced a post-ischaemic long-term synaptic potentiation (i-LTP) at corticostriatal synapses. We compared the physiological and pharmacological characteristics of this pathological form of synaptic plasticity with those of LTP induced by tetanic stimulation of corticostriatal fibres (t-LTP), which is thought to represent a cellular substrate of learning and memory. Activation of N-methyl-D-aspartate (NMDA) receptors was required for the induction of both forms of synaptic plasticity. The intraneuronal injection of the calcium chelator BAPTA [bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetate] and inhibitors of the mitogen-activated protein kinase pathway blocked both forms of synaptic plasticity. However, while t-LTP showed input specificity, i-LTP occurred also at synaptic pathways inactive during the ischaemic period. In addition, scopolamine, a muscarinic receptor antagonist, prevented the induction of t-LTP but not of i-LTP, indicating that endogenous acetylcholine is required for physiological but not for pathological synaptic potentiation. Finally, we found that striatal cholinergic interneurones, which are resistant to in vivo ischaemia, do not express i-LTP while they express t-LTP. We suggest that i-LTP represents a pathological form of synaptic plasticity that may account for the cell type-specific vulnerability observed in striatal spiny neurones following ischaemia and energy deprivation.

Original languageEnglish
Pages (from-to)844-860
Number of pages17
JournalBrain
Volume125
Issue number4
Publication statusPublished - 2002

Fingerprint

Long-Term Potentiation
Neuronal Plasticity
Corpus Striatum
Ischemia
Muscarinic Antagonists
Ethane
Scopolamine Hydrobromide
Muscarinic Receptors
Interneurons
Mitogen-Activated Protein Kinases
N-Methyl-D-Aspartate Receptors
Synapses
Cholinergic Agents
Acetylcholine
Learning
Pharmacology
Oxygen
Neurons
Glucose
Injections

Keywords

  • Excitotoxicity
  • NMDA receptors
  • Oxygen/glucose deprivation
  • Synaptic plasticity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Post-ischaemic long-term synaptic potentiation in the striatum : A putative mechanism for cell type-specific vulnerability. / Calabresi, Paolo; Saulle, Emilia; Centonze, Diego; Pisani, Antonio; Marfia, Girolama A.; Bernardi, Giorgio.

In: Brain, Vol. 125, No. 4, 2002, p. 844-860.

Research output: Contribution to journalArticle

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