Post-natal development and regulation of cerebellar cyclic guanosine monophosphate system

P. F. Spano, K. Kumakura, S. Govoni, M. Trabucchi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Recent investigations suggest a role for cyclic GMP in cerebellum as an important factor involved in the regulation of Purkinje cells activity. Since the formation of synapses in cerebellar Purkinje cell body of rat is complete only after the first 15 days of life, cGMP levels have been measured during development. Young adult and three day old rats have been used. The animals were killed by a beam of focused microwave radiation on the skull for 3 seconds. In the new born rat cerebellum the levels of cGMP are much lower than in adult animals. Harmaline, a potent and selective activator of the climbing fibers, a pathway which activates the onset of burst discharges in the Purkinje cells when injected in adult rats,induces, as it has been-previously shown, a 5 fold increase of the level of cerebellar cGMP. In contrast in new born rats harmaline does not increase cGMP concentrations. Moreover diazepam, which has been shown to decrease cerebellar cGMP levels in adult rats, does not appear to have such effect in new borns. On the other hand guanylate-cyclase activity is significantly higher in the new born rats in comparison with adults. Furthermore Harmaline injection in adult animals induces a stimulation of cerebellar guanylate-cyclase, as measured in vitro.

Original languageEnglish
Pages (from-to)223-237
Number of pages15
JournalPharmacological Research Communications
Volume7
Issue number3
DOIs
Publication statusPublished - 1975

Fingerprint

Cyclic GMP
Harmaline
Purkinje Cells
Guanylate Cyclase
Cerebellum
Diazepam
Microwaves
Skull
Synapses
Young Adult
Radiation
Injections

ASJC Scopus subject areas

  • Pharmacology

Cite this

Post-natal development and regulation of cerebellar cyclic guanosine monophosphate system. / Spano, P. F.; Kumakura, K.; Govoni, S.; Trabucchi, M.

In: Pharmacological Research Communications, Vol. 7, No. 3, 1975, p. 223-237.

Research output: Contribution to journalArticle

Spano, P. F. ; Kumakura, K. ; Govoni, S. ; Trabucchi, M. / Post-natal development and regulation of cerebellar cyclic guanosine monophosphate system. In: Pharmacological Research Communications. 1975 ; Vol. 7, No. 3. pp. 223-237.
@article{28eff1c074ba488b81a8f040cc5da55e,
title = "Post-natal development and regulation of cerebellar cyclic guanosine monophosphate system",
abstract = "Recent investigations suggest a role for cyclic GMP in cerebellum as an important factor involved in the regulation of Purkinje cells activity. Since the formation of synapses in cerebellar Purkinje cell body of rat is complete only after the first 15 days of life, cGMP levels have been measured during development. Young adult and three day old rats have been used. The animals were killed by a beam of focused microwave radiation on the skull for 3 seconds. In the new born rat cerebellum the levels of cGMP are much lower than in adult animals. Harmaline, a potent and selective activator of the climbing fibers, a pathway which activates the onset of burst discharges in the Purkinje cells when injected in adult rats,induces, as it has been-previously shown, a 5 fold increase of the level of cerebellar cGMP. In contrast in new born rats harmaline does not increase cGMP concentrations. Moreover diazepam, which has been shown to decrease cerebellar cGMP levels in adult rats, does not appear to have such effect in new borns. On the other hand guanylate-cyclase activity is significantly higher in the new born rats in comparison with adults. Furthermore Harmaline injection in adult animals induces a stimulation of cerebellar guanylate-cyclase, as measured in vitro.",
author = "Spano, {P. F.} and K. Kumakura and S. Govoni and M. Trabucchi",
year = "1975",
doi = "10.1016/0031-6989(75)90022-3",
language = "English",
volume = "7",
pages = "223--237",
journal = "Pharmacological Research",
issn = "1043-6618",
publisher = "Academic Press",
number = "3",

}

TY - JOUR

T1 - Post-natal development and regulation of cerebellar cyclic guanosine monophosphate system

AU - Spano, P. F.

AU - Kumakura, K.

AU - Govoni, S.

AU - Trabucchi, M.

PY - 1975

Y1 - 1975

N2 - Recent investigations suggest a role for cyclic GMP in cerebellum as an important factor involved in the regulation of Purkinje cells activity. Since the formation of synapses in cerebellar Purkinje cell body of rat is complete only after the first 15 days of life, cGMP levels have been measured during development. Young adult and three day old rats have been used. The animals were killed by a beam of focused microwave radiation on the skull for 3 seconds. In the new born rat cerebellum the levels of cGMP are much lower than in adult animals. Harmaline, a potent and selective activator of the climbing fibers, a pathway which activates the onset of burst discharges in the Purkinje cells when injected in adult rats,induces, as it has been-previously shown, a 5 fold increase of the level of cerebellar cGMP. In contrast in new born rats harmaline does not increase cGMP concentrations. Moreover diazepam, which has been shown to decrease cerebellar cGMP levels in adult rats, does not appear to have such effect in new borns. On the other hand guanylate-cyclase activity is significantly higher in the new born rats in comparison with adults. Furthermore Harmaline injection in adult animals induces a stimulation of cerebellar guanylate-cyclase, as measured in vitro.

AB - Recent investigations suggest a role for cyclic GMP in cerebellum as an important factor involved in the regulation of Purkinje cells activity. Since the formation of synapses in cerebellar Purkinje cell body of rat is complete only after the first 15 days of life, cGMP levels have been measured during development. Young adult and three day old rats have been used. The animals were killed by a beam of focused microwave radiation on the skull for 3 seconds. In the new born rat cerebellum the levels of cGMP are much lower than in adult animals. Harmaline, a potent and selective activator of the climbing fibers, a pathway which activates the onset of burst discharges in the Purkinje cells when injected in adult rats,induces, as it has been-previously shown, a 5 fold increase of the level of cerebellar cGMP. In contrast in new born rats harmaline does not increase cGMP concentrations. Moreover diazepam, which has been shown to decrease cerebellar cGMP levels in adult rats, does not appear to have such effect in new borns. On the other hand guanylate-cyclase activity is significantly higher in the new born rats in comparison with adults. Furthermore Harmaline injection in adult animals induces a stimulation of cerebellar guanylate-cyclase, as measured in vitro.

UR - http://www.scopus.com/inward/record.url?scp=0016812798&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016812798&partnerID=8YFLogxK

U2 - 10.1016/0031-6989(75)90022-3

DO - 10.1016/0031-6989(75)90022-3

M3 - Article

VL - 7

SP - 223

EP - 237

JO - Pharmacological Research

JF - Pharmacological Research

SN - 1043-6618

IS - 3

ER -