Post-transcriptional control of c-myc proto-oncogene expression by glucocorticoid hormones in human T lymphoblastic leukemic cells

M. Maroder, S. Martinotti, A. Vacca, I. Screpanti, E. Petrangeli, L. Frati, A. Gulino

Research output: Contribution to journalArticle

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Abstract

We have studied the regulation of the human c-myc proto-oncogene by glucocorticoid hormones in T lymbhoblastic leukemic cells. A significant decrease (50%) of the steady state levels of c-myc mRNA was observed as early as 3 h after dexamethasone treatment of CEM-1.3 human lymphoma cells, reaching less than 5% values, with respect to untreated cells, 24 h after hormone administration. Nuclear run-on experiments showed no modifications of the transcriptional rate from the first exon. However, a slight decrease (15%) of the transcript elongation from the first exon/first intron boundary was observed in the dexamethasone-treated cells. Using actinomycin D to block gene transcription, we have observed a significant increase in the rate of c-myc RNA specific decay after dexamethasone treatment. Furthermore, cycloheximide was able to overcome completely the dexamethasone-induced down-regulation of the c-myc gene expression. Our data suggest that dexamethasone is able to inhibit human c-myc gene expression primarily at the post-transcriptional level, through the synthesis of hormone-induced regulatory protein(s) controlling c-myc transcript stability.

Original languageEnglish
Pages (from-to)1153-1157
Number of pages5
JournalNucleic Acids Research
Volume18
Issue number5
Publication statusPublished - Mar 11 1990

Fingerprint

myc Genes
Hormones
Dexamethasone
Glucocorticoids
Gene expression
Cell
Gene Expression
Transcription
Exons
RNA
Decrease
Elongation
Genes
Cells
Messenger RNA
Proteins
RNA Stability
Dactinomycin
Cycloheximide
Introns

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Maroder, M., Martinotti, S., Vacca, A., Screpanti, I., Petrangeli, E., Frati, L., & Gulino, A. (1990). Post-transcriptional control of c-myc proto-oncogene expression by glucocorticoid hormones in human T lymphoblastic leukemic cells. Nucleic Acids Research, 18(5), 1153-1157.

Post-transcriptional control of c-myc proto-oncogene expression by glucocorticoid hormones in human T lymphoblastic leukemic cells. / Maroder, M.; Martinotti, S.; Vacca, A.; Screpanti, I.; Petrangeli, E.; Frati, L.; Gulino, A.

In: Nucleic Acids Research, Vol. 18, No. 5, 11.03.1990, p. 1153-1157.

Research output: Contribution to journalArticle

Maroder, M, Martinotti, S, Vacca, A, Screpanti, I, Petrangeli, E, Frati, L & Gulino, A 1990, 'Post-transcriptional control of c-myc proto-oncogene expression by glucocorticoid hormones in human T lymphoblastic leukemic cells', Nucleic Acids Research, vol. 18, no. 5, pp. 1153-1157.
Maroder, M. ; Martinotti, S. ; Vacca, A. ; Screpanti, I. ; Petrangeli, E. ; Frati, L. ; Gulino, A. / Post-transcriptional control of c-myc proto-oncogene expression by glucocorticoid hormones in human T lymphoblastic leukemic cells. In: Nucleic Acids Research. 1990 ; Vol. 18, No. 5. pp. 1153-1157.
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