Postabsorptive muscle protein metabolism in type 1 diabetic patients after pancreas transplantation

L. Luzi, L. Piceni Sereni, M. Spessot, R. Dodesini, M. R. Pastore, M. Bianchi, I. Terruzzi, A. Secchi, M. Cristallo, G. Pozza, V. Di Carlo

Research output: Contribution to journalArticle

Abstract

Insulin was shown to induce protein anabolism in vivo mainly by inhibiting proteolysis. Heterotopic pancreas transplantation in type 1 diabetes mellitus is characterized by peripheral hyperinsulinemia due to systemic rather than portal insulin delivery. Therefore, we studied the postabsorptive muscle protein metabolism in type 1 diabetic patients with or without pancreas transplantation. The forearm balance technique was performed in 9 type 1 diabetic patients on exogenous insulin treatment, in 4 type 1 diabetic patients following successful pancreas transplantation and in 6 healthy volunteers. Labelled leucine and phenylalanine were infused to quantify whole-body and muscle protein synthesis, respectively. In the postabsorptive state, whole-body protein synthesis (leucine kinetics) was similar in pancreas-transplanted patients and controls. In contrast, muscle protein synthesis tended to be less negative in pancreas-transplanted patients with respect to type 1 diabetic patients and healthy volunteers. The present data suggest that recipients with peripheral insulin delivery and chronic hyperinsulinemia are characterized by a preferential stimulation of protein synthesis in muscle rather than in the splanchnic district. When insulin was infused acutely, while maintaining euglycemia, the whole-body and muscle protein synthesis rates were approximately halved in type 1 diabetic patients with and without pancreas transplantation. We conclude that pancreas transplantation is able to normalize basal and insulin-stimulated protein metabolism. Chronic hyperinsulinemia counteract steroid-induced protein degradation by means of a mild, but persistent stimulation of muscle protein synthesis.

Original languageEnglish
Pages (from-to)219-224
Number of pages6
JournalActa Diabetologica
Volume37
Issue number4
DOIs
Publication statusPublished - 2000

Keywords

  • Muscle protein metabolism
  • Pancreas transplantation

ASJC Scopus subject areas

  • Endocrinology
  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

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    Luzi, L., Piceni Sereni, L., Spessot, M., Dodesini, R., Pastore, M. R., Bianchi, M., Terruzzi, I., Secchi, A., Cristallo, M., Pozza, G., & Di Carlo, V. (2000). Postabsorptive muscle protein metabolism in type 1 diabetic patients after pancreas transplantation. Acta Diabetologica, 37(4), 219-224. https://doi.org/10.1007/s005920070009