Postantibiotic effect and delay of regrowth in strains carrying mutations that save proteins or RNA

Marzia Dolcino, Alberto Zoratti, Eugenio A. Debbia, Gian Carlo Schito, Anna Marchese

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The postantibiotic effect (PAE) values found for proteinase-defective (Lon-) Escherichia coli and RNase-defective E. coli exposed to antibiotics were reduced (31 to 60% and 35 to 50%, respectively) in comparison with the control (AB1157), and in the recA13 mutant these values were about 0.4 h with all drugs. Nalidixic acid, under anaerobic conditions, induced no PAE (0 to 0.1 h) in AB1157. A delay in regrowth (0.2 to 0.26 h) was noted with dnaA46(Ts), gyrA43(Ts), and gyrB41 (Ts) mutants cultured for 2 h at 43°C. These findings suggest that when proteins and RNA are saved, the cell rapidly resumes the original growth rate.

Original languageEnglish
Pages (from-to)4022-4025
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume46
Issue number12
DOIs
Publication statusPublished - Dec 1 2002

Fingerprint

RNA
Escherichia coli
Nalidixic Acid
Mutation
Proteins
Peptide Hydrolases
Anti-Bacterial Agents
Growth
Pharmaceutical Preparations
ribonuclease E

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Postantibiotic effect and delay of regrowth in strains carrying mutations that save proteins or RNA. / Dolcino, Marzia; Zoratti, Alberto; Debbia, Eugenio A.; Schito, Gian Carlo; Marchese, Anna.

In: Antimicrobial Agents and Chemotherapy, Vol. 46, No. 12, 01.12.2002, p. 4022-4025.

Research output: Contribution to journalArticle

Dolcino, Marzia ; Zoratti, Alberto ; Debbia, Eugenio A. ; Schito, Gian Carlo ; Marchese, Anna. / Postantibiotic effect and delay of regrowth in strains carrying mutations that save proteins or RNA. In: Antimicrobial Agents and Chemotherapy. 2002 ; Vol. 46, No. 12. pp. 4022-4025.
@article{cd4a7658f2ce4a5eb2c9d04bedb906a2,
title = "Postantibiotic effect and delay of regrowth in strains carrying mutations that save proteins or RNA",
abstract = "The postantibiotic effect (PAE) values found for proteinase-defective (Lon-) Escherichia coli and RNase-defective E. coli exposed to antibiotics were reduced (31 to 60{\%} and 35 to 50{\%}, respectively) in comparison with the control (AB1157), and in the recA13 mutant these values were about 0.4 h with all drugs. Nalidixic acid, under anaerobic conditions, induced no PAE (0 to 0.1 h) in AB1157. A delay in regrowth (0.2 to 0.26 h) was noted with dnaA46(Ts), gyrA43(Ts), and gyrB41 (Ts) mutants cultured for 2 h at 43°C. These findings suggest that when proteins and RNA are saved, the cell rapidly resumes the original growth rate.",
author = "Marzia Dolcino and Alberto Zoratti and Debbia, {Eugenio A.} and Schito, {Gian Carlo} and Anna Marchese",
year = "2002",
month = "12",
day = "1",
doi = "10.1128/AAC.46.12.4022-4025.2002",
language = "English",
volume = "46",
pages = "4022--4025",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "12",

}

TY - JOUR

T1 - Postantibiotic effect and delay of regrowth in strains carrying mutations that save proteins or RNA

AU - Dolcino, Marzia

AU - Zoratti, Alberto

AU - Debbia, Eugenio A.

AU - Schito, Gian Carlo

AU - Marchese, Anna

PY - 2002/12/1

Y1 - 2002/12/1

N2 - The postantibiotic effect (PAE) values found for proteinase-defective (Lon-) Escherichia coli and RNase-defective E. coli exposed to antibiotics were reduced (31 to 60% and 35 to 50%, respectively) in comparison with the control (AB1157), and in the recA13 mutant these values were about 0.4 h with all drugs. Nalidixic acid, under anaerobic conditions, induced no PAE (0 to 0.1 h) in AB1157. A delay in regrowth (0.2 to 0.26 h) was noted with dnaA46(Ts), gyrA43(Ts), and gyrB41 (Ts) mutants cultured for 2 h at 43°C. These findings suggest that when proteins and RNA are saved, the cell rapidly resumes the original growth rate.

AB - The postantibiotic effect (PAE) values found for proteinase-defective (Lon-) Escherichia coli and RNase-defective E. coli exposed to antibiotics were reduced (31 to 60% and 35 to 50%, respectively) in comparison with the control (AB1157), and in the recA13 mutant these values were about 0.4 h with all drugs. Nalidixic acid, under anaerobic conditions, induced no PAE (0 to 0.1 h) in AB1157. A delay in regrowth (0.2 to 0.26 h) was noted with dnaA46(Ts), gyrA43(Ts), and gyrB41 (Ts) mutants cultured for 2 h at 43°C. These findings suggest that when proteins and RNA are saved, the cell rapidly resumes the original growth rate.

UR - http://www.scopus.com/inward/record.url?scp=0036897157&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036897157&partnerID=8YFLogxK

U2 - 10.1128/AAC.46.12.4022-4025.2002

DO - 10.1128/AAC.46.12.4022-4025.2002

M3 - Article

C2 - 12435717

AN - SCOPUS:0036897157

VL - 46

SP - 4022

EP - 4025

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 12

ER -