Postnatal development of the vesicular GABA transporter in rat cerebral cortex

A. Minelli, L. Alonso-Nanclares, R. H. Edwards, J. Defelipe, F. Conti

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Light and electron microscopic immunocytochemical techniques and Western blotting were used to investigate the postnatal development of the vesicular GABA transporter (VGAT) in the rat somatic sensory cortex. VGAT immunoreactivity was low at birth, it increased gradually through the first and second weeks of life and achieved the adult pattern during the third week. At postnatal day (P)0-P5, VGAT immunoreactivity was associated exclusively to fibers and puncta. Electron microscopic studies performed at P5 showed that all identified synaptic contacts formed by VGAT-positive axonal swellings were of the symmetric type and that a substantial proportion of the boutons appeared not to have formed synapses. From P10 onward, labeled puncta were both scattered in the neuropil and in apposition to unstained cellular profiles; VGAT was also expressed in few GABAergic cell bodies. Western blottings at the same postnatal ages revealed a 55-kDa band whose intensity was weak at P0 (17% of adult), it increased constantly until P15 (P2: 35%; P5: 44%; P10: 68%; P15: 97%), and then leveled off. Overall, the present results show that during neocortical development the expression of VGAT slightly precedes the complete maturation of inhibitory synaptogenesis and suggest that it may contribute to the formation of neocortical GABAergic circuitry.

Original languageEnglish
Pages (from-to)337-346
Number of pages10
JournalNeuroscience
Volume117
Issue number2
DOIs
Publication statusPublished - Mar 21 2003

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Cerebral Cortex
Western Blotting
Electrons
Neuropil
Synapses
vesicular GABA transporter
Parturition
Light

Keywords

  • Development
  • GABA
  • GAT-1
  • Neurotransmitter uptake
  • Synaptic vesicles

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Postnatal development of the vesicular GABA transporter in rat cerebral cortex. / Minelli, A.; Alonso-Nanclares, L.; Edwards, R. H.; Defelipe, J.; Conti, F.

In: Neuroscience, Vol. 117, No. 2, 21.03.2003, p. 337-346.

Research output: Contribution to journalArticle

Minelli, A, Alonso-Nanclares, L, Edwards, RH, Defelipe, J & Conti, F 2003, 'Postnatal development of the vesicular GABA transporter in rat cerebral cortex', Neuroscience, vol. 117, no. 2, pp. 337-346. https://doi.org/10.1016/S0306-4522(02)00864-3
Minelli, A. ; Alonso-Nanclares, L. ; Edwards, R. H. ; Defelipe, J. ; Conti, F. / Postnatal development of the vesicular GABA transporter in rat cerebral cortex. In: Neuroscience. 2003 ; Vol. 117, No. 2. pp. 337-346.
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