Postprandial chylomicrons and adipose tissue lipoprotein lipase are altered in type 2 diabetes independently of obesity and whole-body insulin resistance

Giovanni Annuzzi, Rosalba Giacco, Lidia Patti, Lucrezia Di Marino, Claudia De Natale, Giuseppina Costabile, Maurizio Marra, Carmela Santangelo, Roberta Masella, Angela A. Rivellese

Research output: Contribution to journalArticle

Abstract

Background and aims: Postprandial lipoprotein abnormalities in type 2 diabetes are associated with insulin resistance. The role of other diabetes-related factors is still not clear. The aim of this study is to differentiate the effects of whole-body insulin resistance, obesity, and type 2 diabetes on postprandial dyslipidaemia and lipoprotein lipase (LPL) in adipose tissue. Methods and results: Ten subjects with obesity and diabetes (OD), 11 with obesity alone (O), and 11 normal-weight controls (C) - males, aged 26-59 years, with fasting normo-triglyceridaemia underwent measurements of cholesterol, triglycerides, apo B-48 and apo B-100 concentrations in plasma lipoproteins separated by density gradient ultracentrifugation before and after a fat-rich meal. Fasting and postprandial (6 h) LPL activity was determined in abdominal subcutaneous adipose tissue biopsy samples. Insulin sensitivity was measured by hyperinsulinaemic euglycaemic clamp. OD and O subjects had similar degrees of adiposity (BMI, waist circumference, fat mass) and insulin resistance (insulin stimulated glucose disposal and M/I). They also showed a similarly higher postprandial increase in large VLDL lipids (triglyceride incremental AUC 188 ± 28 and 135 ± 22 mg/dl·6 h) than C (87 ± 13 mg/dl·6 h, M ± SEM, p <0.05). OD had an increased chylomicron response compared to O (triglyceride incremental AUC 132 ± 23 vs. 75 ± 14 mg/dl·6 h, p <0.05). OD had significantly lower fasting and postprandial adipose tissue heparin-releasable LPL activity than O and C. Conclusions: In insulin-resistant conditions of obesity, with and without diabetes, large VLDL are increased after a fat-rich meal. In addition, diabetic patients compared to obese subjects have an increased postprandial chylomicron response and a reduced adipose tissue LPL activity.

Original languageEnglish
Pages (from-to)531-538
Number of pages8
JournalNutrition, Metabolism and Cardiovascular Diseases
Volume18
Issue number8
DOIs
Publication statusPublished - Oct 2008

Fingerprint

Chylomicrons
Lipoprotein Lipase
Type 2 Diabetes Mellitus
Insulin Resistance
Adipose Tissue
Obesity
Fasting
Fats
Lipoproteins
Area Under Curve
Meals
Triglycerides
Apolipoprotein B-48
Abdominal Subcutaneous Fat
Insulin
Apolipoprotein B-100
Glucose Clamp Technique
Ultracentrifugation
Adiposity
Waist Circumference

Keywords

  • Adipose tissue
  • Insulin resistance
  • Lipoprotein lipase
  • Postprandial lipoproteins
  • Type 2 diabetes

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Postprandial chylomicrons and adipose tissue lipoprotein lipase are altered in type 2 diabetes independently of obesity and whole-body insulin resistance. / Annuzzi, Giovanni; Giacco, Rosalba; Patti, Lidia; Di Marino, Lucrezia; De Natale, Claudia; Costabile, Giuseppina; Marra, Maurizio; Santangelo, Carmela; Masella, Roberta; Rivellese, Angela A.

In: Nutrition, Metabolism and Cardiovascular Diseases, Vol. 18, No. 8, 10.2008, p. 531-538.

Research output: Contribution to journalArticle

Annuzzi, G, Giacco, R, Patti, L, Di Marino, L, De Natale, C, Costabile, G, Marra, M, Santangelo, C, Masella, R & Rivellese, AA 2008, 'Postprandial chylomicrons and adipose tissue lipoprotein lipase are altered in type 2 diabetes independently of obesity and whole-body insulin resistance', Nutrition, Metabolism and Cardiovascular Diseases, vol. 18, no. 8, pp. 531-538. https://doi.org/10.1016/j.numecd.2007.12.003
Annuzzi, Giovanni ; Giacco, Rosalba ; Patti, Lidia ; Di Marino, Lucrezia ; De Natale, Claudia ; Costabile, Giuseppina ; Marra, Maurizio ; Santangelo, Carmela ; Masella, Roberta ; Rivellese, Angela A. / Postprandial chylomicrons and adipose tissue lipoprotein lipase are altered in type 2 diabetes independently of obesity and whole-body insulin resistance. In: Nutrition, Metabolism and Cardiovascular Diseases. 2008 ; Vol. 18, No. 8. pp. 531-538.
@article{f448d128d1374730b1fdb43c0371322f,
title = "Postprandial chylomicrons and adipose tissue lipoprotein lipase are altered in type 2 diabetes independently of obesity and whole-body insulin resistance",
abstract = "Background and aims: Postprandial lipoprotein abnormalities in type 2 diabetes are associated with insulin resistance. The role of other diabetes-related factors is still not clear. The aim of this study is to differentiate the effects of whole-body insulin resistance, obesity, and type 2 diabetes on postprandial dyslipidaemia and lipoprotein lipase (LPL) in adipose tissue. Methods and results: Ten subjects with obesity and diabetes (OD), 11 with obesity alone (O), and 11 normal-weight controls (C) - males, aged 26-59 years, with fasting normo-triglyceridaemia underwent measurements of cholesterol, triglycerides, apo B-48 and apo B-100 concentrations in plasma lipoproteins separated by density gradient ultracentrifugation before and after a fat-rich meal. Fasting and postprandial (6 h) LPL activity was determined in abdominal subcutaneous adipose tissue biopsy samples. Insulin sensitivity was measured by hyperinsulinaemic euglycaemic clamp. OD and O subjects had similar degrees of adiposity (BMI, waist circumference, fat mass) and insulin resistance (insulin stimulated glucose disposal and M/I). They also showed a similarly higher postprandial increase in large VLDL lipids (triglyceride incremental AUC 188 ± 28 and 135 ± 22 mg/dl·6 h) than C (87 ± 13 mg/dl·6 h, M ± SEM, p <0.05). OD had an increased chylomicron response compared to O (triglyceride incremental AUC 132 ± 23 vs. 75 ± 14 mg/dl·6 h, p <0.05). OD had significantly lower fasting and postprandial adipose tissue heparin-releasable LPL activity than O and C. Conclusions: In insulin-resistant conditions of obesity, with and without diabetes, large VLDL are increased after a fat-rich meal. In addition, diabetic patients compared to obese subjects have an increased postprandial chylomicron response and a reduced adipose tissue LPL activity.",
keywords = "Adipose tissue, Insulin resistance, Lipoprotein lipase, Postprandial lipoproteins, Type 2 diabetes",
author = "Giovanni Annuzzi and Rosalba Giacco and Lidia Patti and {Di Marino}, Lucrezia and {De Natale}, Claudia and Giuseppina Costabile and Maurizio Marra and Carmela Santangelo and Roberta Masella and Rivellese, {Angela A.}",
year = "2008",
month = "10",
doi = "10.1016/j.numecd.2007.12.003",
language = "English",
volume = "18",
pages = "531--538",
journal = "Nutrition, Metabolism and Cardiovascular Diseases",
issn = "0939-4753",
publisher = "Elsevier B.V.",
number = "8",

}

TY - JOUR

T1 - Postprandial chylomicrons and adipose tissue lipoprotein lipase are altered in type 2 diabetes independently of obesity and whole-body insulin resistance

AU - Annuzzi, Giovanni

AU - Giacco, Rosalba

AU - Patti, Lidia

AU - Di Marino, Lucrezia

AU - De Natale, Claudia

AU - Costabile, Giuseppina

AU - Marra, Maurizio

AU - Santangelo, Carmela

AU - Masella, Roberta

AU - Rivellese, Angela A.

PY - 2008/10

Y1 - 2008/10

N2 - Background and aims: Postprandial lipoprotein abnormalities in type 2 diabetes are associated with insulin resistance. The role of other diabetes-related factors is still not clear. The aim of this study is to differentiate the effects of whole-body insulin resistance, obesity, and type 2 diabetes on postprandial dyslipidaemia and lipoprotein lipase (LPL) in adipose tissue. Methods and results: Ten subjects with obesity and diabetes (OD), 11 with obesity alone (O), and 11 normal-weight controls (C) - males, aged 26-59 years, with fasting normo-triglyceridaemia underwent measurements of cholesterol, triglycerides, apo B-48 and apo B-100 concentrations in plasma lipoproteins separated by density gradient ultracentrifugation before and after a fat-rich meal. Fasting and postprandial (6 h) LPL activity was determined in abdominal subcutaneous adipose tissue biopsy samples. Insulin sensitivity was measured by hyperinsulinaemic euglycaemic clamp. OD and O subjects had similar degrees of adiposity (BMI, waist circumference, fat mass) and insulin resistance (insulin stimulated glucose disposal and M/I). They also showed a similarly higher postprandial increase in large VLDL lipids (triglyceride incremental AUC 188 ± 28 and 135 ± 22 mg/dl·6 h) than C (87 ± 13 mg/dl·6 h, M ± SEM, p <0.05). OD had an increased chylomicron response compared to O (triglyceride incremental AUC 132 ± 23 vs. 75 ± 14 mg/dl·6 h, p <0.05). OD had significantly lower fasting and postprandial adipose tissue heparin-releasable LPL activity than O and C. Conclusions: In insulin-resistant conditions of obesity, with and without diabetes, large VLDL are increased after a fat-rich meal. In addition, diabetic patients compared to obese subjects have an increased postprandial chylomicron response and a reduced adipose tissue LPL activity.

AB - Background and aims: Postprandial lipoprotein abnormalities in type 2 diabetes are associated with insulin resistance. The role of other diabetes-related factors is still not clear. The aim of this study is to differentiate the effects of whole-body insulin resistance, obesity, and type 2 diabetes on postprandial dyslipidaemia and lipoprotein lipase (LPL) in adipose tissue. Methods and results: Ten subjects with obesity and diabetes (OD), 11 with obesity alone (O), and 11 normal-weight controls (C) - males, aged 26-59 years, with fasting normo-triglyceridaemia underwent measurements of cholesterol, triglycerides, apo B-48 and apo B-100 concentrations in plasma lipoproteins separated by density gradient ultracentrifugation before and after a fat-rich meal. Fasting and postprandial (6 h) LPL activity was determined in abdominal subcutaneous adipose tissue biopsy samples. Insulin sensitivity was measured by hyperinsulinaemic euglycaemic clamp. OD and O subjects had similar degrees of adiposity (BMI, waist circumference, fat mass) and insulin resistance (insulin stimulated glucose disposal and M/I). They also showed a similarly higher postprandial increase in large VLDL lipids (triglyceride incremental AUC 188 ± 28 and 135 ± 22 mg/dl·6 h) than C (87 ± 13 mg/dl·6 h, M ± SEM, p <0.05). OD had an increased chylomicron response compared to O (triglyceride incremental AUC 132 ± 23 vs. 75 ± 14 mg/dl·6 h, p <0.05). OD had significantly lower fasting and postprandial adipose tissue heparin-releasable LPL activity than O and C. Conclusions: In insulin-resistant conditions of obesity, with and without diabetes, large VLDL are increased after a fat-rich meal. In addition, diabetic patients compared to obese subjects have an increased postprandial chylomicron response and a reduced adipose tissue LPL activity.

KW - Adipose tissue

KW - Insulin resistance

KW - Lipoprotein lipase

KW - Postprandial lipoproteins

KW - Type 2 diabetes

UR - http://www.scopus.com/inward/record.url?scp=40749084230&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40749084230&partnerID=8YFLogxK

U2 - 10.1016/j.numecd.2007.12.003

DO - 10.1016/j.numecd.2007.12.003

M3 - Article

C2 - 18321693

AN - SCOPUS:40749084230

VL - 18

SP - 531

EP - 538

JO - Nutrition, Metabolism and Cardiovascular Diseases

JF - Nutrition, Metabolism and Cardiovascular Diseases

SN - 0939-4753

IS - 8

ER -