Posttranscriptional control of human gamma interferon gene expression in transfected mouse fibroblasts.

H. A. Young, L. Varesio, P. Hwu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Human gamma interferon genomic DNA was introduced into NIH 3T3 fibroblasts by calcium phosphate precipitation and was not expressed in these cells at the cytoplasmic mRNA or protein level. Treatment of the transfected cells with cycloheximide (1 microgram/ml) induced the accumulation of cytoplasmic gamma interferon mRNA and biologically active human gamma interferon. Analysis of the nuclear enriched RNA from untreated cells indicated that human gamma interferon mRNA was present, suggesting that cycloheximide may act by inhibiting a specific nuclease or may enhance the processing or transport of the RNA from the nucleus to the cytoplasm.

Original languageEnglish
Pages (from-to)2253-2256
Number of pages4
JournalMolecular and Cellular Biology
Volume6
Issue number6
Publication statusPublished - Jun 1986

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Fibroblasts
Cycloheximide
Gene Expression
Messenger RNA
RNA Transport
Nuclear RNA
Interferon-gamma
Cytoplasm
DNA
human IFNG protein
Proteins
calcium phosphate

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Posttranscriptional control of human gamma interferon gene expression in transfected mouse fibroblasts. / Young, H. A.; Varesio, L.; Hwu, P.

In: Molecular and Cellular Biology, Vol. 6, No. 6, 06.1986, p. 2253-2256.

Research output: Contribution to journalArticle

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