Posttranscriptional stimulation of endothelial cell matrix metalloproteinases 2 and 1 by endothelioma cells

Giulia Taraboletti, Laura Sonzogni, Veronica Vergani, Ghamartaj Hosseini, Roberta Ceruti, Carmen Ghilardi, Antonio Bastone, Elena Toschi, Patrizia Borsotti, Eugenio Scanziani, Raffaella Giavazzi, Michael S. Pepper, William G. Stetler-Stevenson, Maria Rosa Bani

Research output: Contribution to journalArticlepeer-review


Matrix metalloproteinases (MMPs) play a critical role in the development of hemangioma-like vascular tumors in mice injected with routine eEnd.1 endothelioma cells. The current study was designed to (a) characterize the presence of MMPs in the vascular tumor, (b) define whether these MMPs originate from the transformed cells or from the recruited stromal cells and (c) study the stimulatory effect of eEnd.1 cells on the production of MMPs by endothelial cells. Several gelatinases were present in the eEnd.1 tumor extract, including latent and activated MMP-2 (72-kDa gelatinase A, EC and MMP-9 (92-kDa gelatinase B, EC Immunohistochemical analysis of the tumor revealed focal reactivity for MMP-2. No gelatinase was produced by cultured eEnd.1 cells, or by six of nine related endothelioma cell lines, suggesting that stroma cells, particularly endothelial cells recruited by the tumor cells, rather than eEnd.1 cells themselves, are the source of the gelatinases observed in the tumors in vivo. The conditioned medium of eEnd.1 cells stimulated the release of MMP-2 and MMP-1 (interstitial collagenase, EC by endothelial cells, but not of the inhibitor TIMP-2. The increased production of MMP-2 and MMP-1, observed at the protein level (zymogram and Western blot analysis), occurred through a posttranscriptional mechanism, since no increase in mRNA was observed and the stimulation was not prevented by inhibitors of protein synthesis. The inhibitory effects of monensin and brefeldin A, in inhibitors of protein secretion, and the decrease in cell-associated MMP-2 in stimulated endothelial cells indicated that regulation occurred mostly at the level of protease secretion. MMPs are known to be regulated at different levels; this study indicates that in endothelial cells, the stimulation of MMPs can also occur at the level of secretion, a mechanism that provides a rapid mobilization of these crucial enzymes in the early phases of angiogenesis. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)384-394
Number of pages11
JournalExperimental Cell Research
Issue number2
Publication statusPublished - Aug 1 2000


  • Angiogenesis
  • Endothelial cells
  • Endothelioma
  • Hemangioma
  • Matrix metalloproteinases
  • Proteases secretion

ASJC Scopus subject areas

  • Cell Biology


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