Potential anti-inflammatory effects of the hydrophilic fraction of pomegranate (Punica granatum L.) Seed oil on breast cancer cell lines

Susan Costantini, Fabiola Rusolo, Valentina De Vito, Stefania Moccia, Gianluca Picariello, Francesca Capone, Eliana Guerriero, Giuseppe Castello, Maria Grazia Volpe

Research output: Contribution to journalArticle

Abstract

In this work, we characterized conjugated linolenic acids (e.g., punicic acid) as the major components of the hydrophilic fraction (80% aqueous methanol extract) from pomegranate (Punica granatum L.) seed oil (PSO) and evaluated their anti-inflammatory potential on some human colon (HT29 and HCT116), liver (HepG2 and Huh7), breast (MCF-7 and MDA-MB-231) and prostate (DU145) cancer lines. Our results demonstrated that punicic acid and its congeners induce a significant decrease of cell viability for two breast cell lines with a related increase of the cell cycle G0/G1 phase respect to untreated cells. Moreover, the evaluation of a great panel of cytokines expressed by MCF-7 and MDA-MB-231 cells showed that the levels of VEGF and nine pro-inflammatory cytokines (IL-2, IL-6, IL-12, IL-17, IP-10, MIP-1α, MIP-1β, MCP-1 and TNF-α) decreased in a dose dependent way with increasing amounts of the hydrophilic extracts of PSO, supporting the evidence of an anti-inflammatory effect. Taken together, the data herein suggest a potential synergistic cytotoxic, anti-inflammatory and anti-oxidant role of the polar compounds from PSO.

Original languageEnglish
Pages (from-to)8644-8660
Number of pages17
JournalMolecules
Volume19
Issue number6
DOIs
Publication statusPublished - 2014

Keywords

  • Anti-inflammatory effects
  • Bioactive molecules
  • Cell viability
  • Cytokines
  • Pomegranate (Punica granatum L.)

ASJC Scopus subject areas

  • Organic Chemistry
  • Medicine(all)

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  • Cite this

    Costantini, S., Rusolo, F., De Vito, V., Moccia, S., Picariello, G., Capone, F., Guerriero, E., Castello, G., & Volpe, M. G. (2014). Potential anti-inflammatory effects of the hydrophilic fraction of pomegranate (Punica granatum L.) Seed oil on breast cancer cell lines. Molecules, 19(6), 8644-8660. https://doi.org/10.3390/molecules19068644