TY - JOUR
T1 - Potential anti-inflammatory effects of the hydrophilic fraction of pomegranate (Punica granatum L.) Seed oil on breast cancer cell lines
AU - Costantini, Susan
AU - Rusolo, Fabiola
AU - De Vito, Valentina
AU - Moccia, Stefania
AU - Picariello, Gianluca
AU - Capone, Francesca
AU - Guerriero, Eliana
AU - Castello, Giuseppe
AU - Volpe, Maria Grazia
PY - 2014
Y1 - 2014
N2 - In this work, we characterized conjugated linolenic acids (e.g., punicic acid) as the major components of the hydrophilic fraction (80% aqueous methanol extract) from pomegranate (Punica granatum L.) seed oil (PSO) and evaluated their anti-inflammatory potential on some human colon (HT29 and HCT116), liver (HepG2 and Huh7), breast (MCF-7 and MDA-MB-231) and prostate (DU145) cancer lines. Our results demonstrated that punicic acid and its congeners induce a significant decrease of cell viability for two breast cell lines with a related increase of the cell cycle G0/G1 phase respect to untreated cells. Moreover, the evaluation of a great panel of cytokines expressed by MCF-7 and MDA-MB-231 cells showed that the levels of VEGF and nine pro-inflammatory cytokines (IL-2, IL-6, IL-12, IL-17, IP-10, MIP-1α, MIP-1β, MCP-1 and TNF-α) decreased in a dose dependent way with increasing amounts of the hydrophilic extracts of PSO, supporting the evidence of an anti-inflammatory effect. Taken together, the data herein suggest a potential synergistic cytotoxic, anti-inflammatory and anti-oxidant role of the polar compounds from PSO.
AB - In this work, we characterized conjugated linolenic acids (e.g., punicic acid) as the major components of the hydrophilic fraction (80% aqueous methanol extract) from pomegranate (Punica granatum L.) seed oil (PSO) and evaluated their anti-inflammatory potential on some human colon (HT29 and HCT116), liver (HepG2 and Huh7), breast (MCF-7 and MDA-MB-231) and prostate (DU145) cancer lines. Our results demonstrated that punicic acid and its congeners induce a significant decrease of cell viability for two breast cell lines with a related increase of the cell cycle G0/G1 phase respect to untreated cells. Moreover, the evaluation of a great panel of cytokines expressed by MCF-7 and MDA-MB-231 cells showed that the levels of VEGF and nine pro-inflammatory cytokines (IL-2, IL-6, IL-12, IL-17, IP-10, MIP-1α, MIP-1β, MCP-1 and TNF-α) decreased in a dose dependent way with increasing amounts of the hydrophilic extracts of PSO, supporting the evidence of an anti-inflammatory effect. Taken together, the data herein suggest a potential synergistic cytotoxic, anti-inflammatory and anti-oxidant role of the polar compounds from PSO.
KW - Anti-inflammatory effects
KW - Bioactive molecules
KW - Cell viability
KW - Cytokines
KW - Pomegranate (Punica granatum L.)
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U2 - 10.3390/molecules19068644
DO - 10.3390/molecules19068644
M3 - Article
C2 - 24962397
AN - SCOPUS:84903270439
VL - 19
SP - 8644
EP - 8660
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 6
ER -