Potential artifact for the increase of tumor associated antigens in serum samples from patients injected with monoclonal antibodies

P. Ferroni, D. E. Milenic, M. Roselli, J. A. Carrasquillo, A. Raubitschek, J. Schlom, D. Colcher

Research output: Contribution to journalArticlepeer-review

Abstract

The administration of [131I]B72.3 MAb for both diagnostic and therapeutic purposes is followed by the development of human anti-mouse antibodies (HAMA) in approx. 70% of patients receiving i.v. doses of MAb above 1 mg. The presence of HAMA in circulation interferes in the detection of the tumor-associated glycoprotein TAG-72 using the CA 72-4 RIA. We have reported elsewhere (Ferroni et al., 1990) that heat treatment at 90 °C, pH 6.5, for 15 min is capable of precipitating and/or inactivating HAMA without causing any significant loss in antigen recovery. Furthermore, the removal of the interfering activity leads to a more correct evaluation of TAG-72 serum levels in HAMA-positive serum samples and to a continued use of TAG-72 in the follow-up of patients undergoing MAb-based clinical protocols.

Original languageEnglish
Pages (from-to)383-387
Number of pages5
JournalInternational Journal of Radiation Applications and Instrumentation.
Volume18
Issue number4
DOIs
Publication statusPublished - 1991

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