TY - JOUR
T1 - Potential associations among alteration of salivary mirnas, saliva microbiome structure, and cognitive impairments in autistic children
AU - Ragusa, Marco
AU - Santagati, Maria
AU - Mirabella, Federica
AU - Lauretta, Giovanni
AU - Cirnigliaro, Matilde
AU - Brex, Duilia
AU - Barbagallo, Cristina
AU - Domini, Carla Noemi
AU - Gulisano, Mariangela
AU - Barone, Rita
AU - Trovato, Laura
AU - Oliveri, Salvatore
AU - Mongelli, Gino
AU - Spitale, Ambra
AU - Barbagallo, Davide
AU - Di Pietro, Cinzia
AU - Stefani, Stefania
AU - Rizzo, Renata
AU - Purrello, Michele
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Recent evidence has demonstrated that salivary molecules, as well as bacterial populations, can be perturbed by several pathological conditions, including neuro-psychiatric diseases. This relationship between brain functionality and saliva composition could be exploited to unveil new pathological mechanisms of elusive diseases, such as Autistic Spectrum Disorder (ASD). We performed a combined approach of miRNA expression profiling by NanoString technology, followed by validation experiments in qPCR, and 16S rRNA microbiome analysis on saliva from 53 ASD and 27 neurologically unaffected control (NUC) children. MiR-29a-3p and miR-141-3p were upregulated, while miR-16-5p, let-7b-5p, and miR-451a were downregulated in ASD compared to NUCs. Microbiome analysis on the same subjects revealed that Rothia, Filifactor, Actinobacillus, Weeksellaceae, Ralstonia, Pasteurellaceae, and Aggregatibacter increased their abundance in ASD patients, while Tannerella, Moryella and TM7-3 decreased. Variations of both miRNAs and microbes were statistically associated to different neuropsychological scores related to anomalies in social interaction and communication. Among miRNA/bacteria associations, the most relevant was the negative correlation between salivary miR-141-3p expression and Tannerella abundance. MiRNA and microbiome dysregulations found in the saliva of ASD children are potentially associated with cognitive impairments of the subjects. Furthermore, a potential cross-talking between circulating miRNAs and resident bacteria could occur in saliva of ASD.
AB - Recent evidence has demonstrated that salivary molecules, as well as bacterial populations, can be perturbed by several pathological conditions, including neuro-psychiatric diseases. This relationship between brain functionality and saliva composition could be exploited to unveil new pathological mechanisms of elusive diseases, such as Autistic Spectrum Disorder (ASD). We performed a combined approach of miRNA expression profiling by NanoString technology, followed by validation experiments in qPCR, and 16S rRNA microbiome analysis on saliva from 53 ASD and 27 neurologically unaffected control (NUC) children. MiR-29a-3p and miR-141-3p were upregulated, while miR-16-5p, let-7b-5p, and miR-451a were downregulated in ASD compared to NUCs. Microbiome analysis on the same subjects revealed that Rothia, Filifactor, Actinobacillus, Weeksellaceae, Ralstonia, Pasteurellaceae, and Aggregatibacter increased their abundance in ASD patients, while Tannerella, Moryella and TM7-3 decreased. Variations of both miRNAs and microbes were statistically associated to different neuropsychological scores related to anomalies in social interaction and communication. Among miRNA/bacteria associations, the most relevant was the negative correlation between salivary miR-141-3p expression and Tannerella abundance. MiRNA and microbiome dysregulations found in the saliva of ASD children are potentially associated with cognitive impairments of the subjects. Furthermore, a potential cross-talking between circulating miRNAs and resident bacteria could occur in saliva of ASD.
KW - ASD
KW - Correlations
KW - Dysbiosis
KW - Illumina
KW - MicroRNA
KW - Nanostring
KW - Oral cavity
KW - Oral microbiota
KW - TaqMan assays
UR - http://www.scopus.com/inward/record.url?scp=85090104208&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85090104208&partnerID=8YFLogxK
U2 - 10.3390/ijms21176203
DO - 10.3390/ijms21176203
M3 - Article
C2 - 32867322
AN - SCOPUS:85090104208
VL - 21
SP - 1
EP - 24
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 17
M1 - 6203
ER -