Potential immunoregulatory role of heme oxygenase-1 in human milk: A combined biochemical and molecular modeling approach

Giovanni Li Volti, Fabio Galvano, Alessandro Frigiola, Salvatore Guccione, Claudia Di Giacomo, Stefano Forte, Giovanni Tringali, Massimo Caruso, Olayiwola Adedotun Adekoya, Diego Gazzolo

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Human milk contains biological factors that are involved in a newborn's growth and immune system regulation. By integrating standard biochemical experimental protocols with computational methods, the present study investigates the presence of heme oxygenase-1 (HO-1), a cytoprotective enzyme, in human milk at different levels of maturation and in milk formulae. Furthermore, we evaluated cytokine and glutathione S-transferase (GSH) levels. Samples were collected from colostrum (on Day 1 after birth), from transition milk (on Postdelivery Days 7 and 14) and from mature milk (on Day 30 after delivery) in 14 healthy women. HO-1 protein, GSH and cytokines levels were measured using enzyme-linked immunosorbent assay and flow cytometry. HO-1 protein levels were significantly higher in colostrum (1.33 ng/ml; 5th centile 0.92; 95th centile 2.38) and in transition milk at 14 days (0.97 ng/ml; 5th centile 0.87; 95th centile 1.45) than in mature milk (0.9 ng/ml; 5th centile 0.8; 95th centile 1.38). Levels of HO-1 in milk formulae were similar to those in colostrum. No significant differences in GSH content were observed in mature milk, transition milk and colostrum, whereas significantly higher GSH levels were observed in milk formulae. No significant levels of cytokines, with the exception of interleukin-8, were found. Computational studies on the possible interactions between HO-1 and CD91 were carried out by a battery of softwares, namely, GRAMM (version 1.03), DALI, CLUSTALW (version 2.0), PatchDock and FireDock, mutually counterchecking and validating each other. The computational results, the strong convergence (to the same "solution") of which finally leads to an "experimental-like" character, showed that HO-1 may bind to CD91, thus suggesting its major role as a new chaperokine in immune response regulation. These findings, which connect and integrate biochemical data and computational data interpretation, represent a synergistic and powerful means of conducting biological research.

Original languageEnglish
Pages (from-to)865-871
Number of pages7
JournalJournal of Nutritional Biochemistry
Volume21
Issue number9
DOIs
Publication statusPublished - Sep 2010

Fingerprint

Heme Oxygenase-1
Molecular modeling
Human Milk
Milk
Colostrum
Cytokines
Immunosorbents
Flow cytometry
Immune system
Biological Factors
Enzymes
Computational methods
Glutathione Transferase
Interleukin-8
Immune System
Assays
Flow Cytometry
Proteins
Software
Enzyme-Linked Immunosorbent Assay

Keywords

  • Glutathione
  • Heat shock proteins
  • Heme oxygenase
  • Human milk
  • Molecular modeling
  • Newborns

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Potential immunoregulatory role of heme oxygenase-1 in human milk : A combined biochemical and molecular modeling approach. / Li Volti, Giovanni; Galvano, Fabio; Frigiola, Alessandro; Guccione, Salvatore; Di Giacomo, Claudia; Forte, Stefano; Tringali, Giovanni; Caruso, Massimo; Adekoya, Olayiwola Adedotun; Gazzolo, Diego.

In: Journal of Nutritional Biochemistry, Vol. 21, No. 9, 09.2010, p. 865-871.

Research output: Contribution to journalArticle

Li Volti, G, Galvano, F, Frigiola, A, Guccione, S, Di Giacomo, C, Forte, S, Tringali, G, Caruso, M, Adekoya, OA & Gazzolo, D 2010, 'Potential immunoregulatory role of heme oxygenase-1 in human milk: A combined biochemical and molecular modeling approach', Journal of Nutritional Biochemistry, vol. 21, no. 9, pp. 865-871. https://doi.org/10.1016/j.jnutbio.2009.06.011
Li Volti, Giovanni ; Galvano, Fabio ; Frigiola, Alessandro ; Guccione, Salvatore ; Di Giacomo, Claudia ; Forte, Stefano ; Tringali, Giovanni ; Caruso, Massimo ; Adekoya, Olayiwola Adedotun ; Gazzolo, Diego. / Potential immunoregulatory role of heme oxygenase-1 in human milk : A combined biochemical and molecular modeling approach. In: Journal of Nutritional Biochemistry. 2010 ; Vol. 21, No. 9. pp. 865-871.
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