Potential impact of a microarray-based nucleic acid assay for rapid detection of gram-negative bacteria and resistance markers in positive blood cultures

Nicasio Mancini, Laura Infurnari, Nadia Ghidoli, Grazia Valzano, Nicola Clementi, Roberto Burioni, Massimo Clementi

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We evaluated the Verigene Gram-negative blood culture (BC-GN) test, a microarray that detects Gram-negative bacteria and several resistance genes. A total of 102 positive blood cultures were tested, and the BC-GN test correctly identified 97.9% of the isolates within its panel. Resistance genes (CTX-M, KPC, VIM, and OXA genes) were detected in 29.8% of the isolates, with positive predictive values of 95.8% (95% confidence interval [CI], 87.7% to 98.9%) in Enterobacteriaceae and 100% (95% CI, 75.9% to 100%) in Pseudomonas aeruginosa and negative predictive values of 100% (95% CI, 93.9% to 100%) and 78.6% (95% CI, 51.0% to 93.6%), respectively.

Original languageEnglish
Pages (from-to)1242-1245
Number of pages4
JournalJournal of Clinical Microbiology
Volume52
Issue number4
DOIs
Publication statusPublished - 2014

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Gram-Negative Bacteria
Nucleic Acids
Confidence Intervals
Genes
Enterobacteriaceae
Pseudomonas aeruginosa
Blood Culture

ASJC Scopus subject areas

  • Microbiology (medical)
  • Medicine(all)

Cite this

Potential impact of a microarray-based nucleic acid assay for rapid detection of gram-negative bacteria and resistance markers in positive blood cultures. / Mancini, Nicasio; Infurnari, Laura; Ghidoli, Nadia; Valzano, Grazia; Clementi, Nicola; Burioni, Roberto; Clementi, Massimo.

In: Journal of Clinical Microbiology, Vol. 52, No. 4, 2014, p. 1242-1245.

Research output: Contribution to journalArticle

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