Abstract
Objective. IL-9 has been shown to be upregulated before the clinical onset of articular disease in RA. The exact role of IL-9 and Th9 cells in RA, however, has not yet been adequately studied. The aim of this study was to evaluate the expression of IL-9 and IL-9-expressing cells in RA patients. Methods. IL-9, IL-9R, PU.1, IL-9, thymic stromal lymphopoietin (TSLP), IL-4 and TGF-β expression was assessed by real-time-PCR in the synovial tissues of RA and OA patients. IL-9, IL-9R, IL-4, TSLP and TGF-β were also investigated by immunohistochemistry. Peripheral CD4+ T cell subsets were studied by flow cytometry analysis before and after incubation with citrullinated peptides. Results. IL-9 was overexpressed in RA synovial tissues and correlated with the degree of histological organization of B and T cells in ectopic lymphoid structures. The majority of IL-9-producing cells were identified as CD3+ cells. Increased mRNA and protein expression of IL-9R, IL-4, TSLP and TGF-β was also observed in RA synovial tissue. Blood peripheral Th9 cells were expanded by citrullinated peptides. Conclusion. These results indicate that Th9 cells and IL-9 were frequently detected in peripheral blood mononuclear cells and synovia of RA patients. A possible pathogenic role for Th9 in RA is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 2264-2272 |
| Number of pages | 9 |
| Journal | Rheumatology |
| Volume | 54 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - 2015 |
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Keywords
- Citrullinated peptide
- IL-9
- Rheumatoid arthritis
- Th9 cells
ASJC Scopus subject areas
- Rheumatology
- Medicine(all)
- Pharmacology (medical)
Cite this
Potential involvement of IL-9 and Th9 cells in the pathogenesis of rheumatoid arthritis. / Ciccia, Francesco; Guggino, Giuliana; Rizzo, Aroldo; Manzo, Antonio; Vitolo, Barbara; Manna, Marco Pio La; Giardina, Giuseppina; Sireci, Guido; Dieli, Francesco; Montecucco, Carlo Maurizio; Alessandro, Riccardo; Triolo, Giovanni.
In: Rheumatology, Vol. 54, No. 12, 2015, p. 2264-2272.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Potential involvement of IL-9 and Th9 cells in the pathogenesis of rheumatoid arthritis
AU - Ciccia, Francesco
AU - Guggino, Giuliana
AU - Rizzo, Aroldo
AU - Manzo, Antonio
AU - Vitolo, Barbara
AU - Manna, Marco Pio La
AU - Giardina, Giuseppina
AU - Sireci, Guido
AU - Dieli, Francesco
AU - Montecucco, Carlo Maurizio
AU - Alessandro, Riccardo
AU - Triolo, Giovanni
PY - 2015
Y1 - 2015
N2 - Objective. IL-9 has been shown to be upregulated before the clinical onset of articular disease in RA. The exact role of IL-9 and Th9 cells in RA, however, has not yet been adequately studied. The aim of this study was to evaluate the expression of IL-9 and IL-9-expressing cells in RA patients. Methods. IL-9, IL-9R, PU.1, IL-9, thymic stromal lymphopoietin (TSLP), IL-4 and TGF-β expression was assessed by real-time-PCR in the synovial tissues of RA and OA patients. IL-9, IL-9R, IL-4, TSLP and TGF-β were also investigated by immunohistochemistry. Peripheral CD4+ T cell subsets were studied by flow cytometry analysis before and after incubation with citrullinated peptides. Results. IL-9 was overexpressed in RA synovial tissues and correlated with the degree of histological organization of B and T cells in ectopic lymphoid structures. The majority of IL-9-producing cells were identified as CD3+ cells. Increased mRNA and protein expression of IL-9R, IL-4, TSLP and TGF-β was also observed in RA synovial tissue. Blood peripheral Th9 cells were expanded by citrullinated peptides. Conclusion. These results indicate that Th9 cells and IL-9 were frequently detected in peripheral blood mononuclear cells and synovia of RA patients. A possible pathogenic role for Th9 in RA is discussed.
AB - Objective. IL-9 has been shown to be upregulated before the clinical onset of articular disease in RA. The exact role of IL-9 and Th9 cells in RA, however, has not yet been adequately studied. The aim of this study was to evaluate the expression of IL-9 and IL-9-expressing cells in RA patients. Methods. IL-9, IL-9R, PU.1, IL-9, thymic stromal lymphopoietin (TSLP), IL-4 and TGF-β expression was assessed by real-time-PCR in the synovial tissues of RA and OA patients. IL-9, IL-9R, IL-4, TSLP and TGF-β were also investigated by immunohistochemistry. Peripheral CD4+ T cell subsets were studied by flow cytometry analysis before and after incubation with citrullinated peptides. Results. IL-9 was overexpressed in RA synovial tissues and correlated with the degree of histological organization of B and T cells in ectopic lymphoid structures. The majority of IL-9-producing cells were identified as CD3+ cells. Increased mRNA and protein expression of IL-9R, IL-4, TSLP and TGF-β was also observed in RA synovial tissue. Blood peripheral Th9 cells were expanded by citrullinated peptides. Conclusion. These results indicate that Th9 cells and IL-9 were frequently detected in peripheral blood mononuclear cells and synovia of RA patients. A possible pathogenic role for Th9 in RA is discussed.
KW - Citrullinated peptide
KW - IL-9
KW - Rheumatoid arthritis
KW - Th9 cells
UR - http://www.scopus.com/inward/record.url?scp=84983168584&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84983168584&partnerID=8YFLogxK
U2 - 10.1093/rheumatology/kev252
DO - 10.1093/rheumatology/kev252
M3 - Article
C2 - 26178600
AN - SCOPUS:84983168584
VL - 54
SP - 2264
EP - 2272
JO - Rheumatology
JF - Rheumatology
SN - 1462-0324
IS - 12
ER -